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Virtual Hematoxylin and Eosin Transillumination Microscopy Using Epi-Fluorescence Imaging

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  • Michael G Giacomelli
  • Lennart Husvogt
  • Hilde Vardeh
  • Beverly E Faulkner-Jones
  • Joachim Hornegger
  • James L Connolly
  • James G Fujimoto

Abstract

We derive a physically realistic model for the generation of virtual transillumination, white light microscopy images using epi-fluorescence measurements from thick, unsectioned tissue. We demonstrate this technique by generating virtual transillumination H&E images of unsectioned human breast tissue from epi-fluorescence multiphoton microscopy data. The virtual transillumination algorithm is shown to enable improved contrast and color accuracy compared with previous color mapping methods. Finally, we present an open source implementation of the algorithm in OpenGL, enabling real-time GPU-based generation of virtual transillumination microscopy images using conventional fluorescence microscopy systems.

Suggested Citation

  • Michael G Giacomelli & Lennart Husvogt & Hilde Vardeh & Beverly E Faulkner-Jones & Joachim Hornegger & James L Connolly & James G Fujimoto, 2016. "Virtual Hematoxylin and Eosin Transillumination Microscopy Using Epi-Fluorescence Imaging," PLOS ONE, Public Library of Science, vol. 11(8), pages 1-13, August.
    • Handle: RePEc:plo:pone00:0159337
    DOI: 10.1371/journal.pone.0159337
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    Cited by:

    1. Lingbo Jin & Yubo Tang & Jackson B. Coole & Melody T. Tan & Xuan Zhao & Hawraa Badaoui & Jacob T. Robinson & Michelle D. Williams & Nadarajah Vigneswaran & Ann M. Gillenwater & Rebecca R. Richards-Kor, 2024. "DeepDOF-SE: affordable deep-learning microscopy platform for slide-free histology," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    2. Matthew T. Martell & Nathaniel J. M. Haven & Brendyn D. Cikaluk & Brendon S. Restall & Ewan A. McAlister & Rohan Mittal & Benjamin A. Adam & Nadia Giannakopoulos & Lashan Peiris & Sveta Silverman & Je, 2023. "Deep learning-enabled realistic virtual histology with ultraviolet photoacoustic remote sensing microscopy," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

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