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Monitoring of Water Spectral Pattern Reveals Differences in Probiotics Growth When Used for Rapid Bacteria Selection

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  • Aleksandar Slavchev
  • Zoltan Kovacs
  • Haruki Koshiba
  • Airi Nagai
  • György Bázár
  • Albert Krastanov
  • Yousuke Kubota
  • Roumiana Tsenkova

Abstract

Development of efficient screening method coupled with cell functionality evaluation is highly needed in contemporary microbiology. The presented novel concept and fast non-destructive method brings in to play the water spectral pattern of the solution as a molecular fingerprint of the cell culture system. To elucidate the concept, NIR spectroscopy with Aquaphotomics were applied to monitor the growth of sixteen Lactobacillus bulgaricus one Lactobacillus pentosus and one Lactobacillus gasseri bacteria strains. Their growth rate, maximal optical density, low pH and bile tolerances were measured and further used as a reference data for analysis of the simultaneously acquired spectral data. The acquired spectral data in the region of 1100-1850nm was subjected to various multivariate data analyses – PCA, OPLS-DA, PLSR. The results showed high accuracy of bacteria strains classification according to their probiotic strength. Most informative spectral fingerprints covered the first overtone of water, emphasizing the relation of water molecular system to cell functionality.

Suggested Citation

  • Aleksandar Slavchev & Zoltan Kovacs & Haruki Koshiba & Airi Nagai & György Bázár & Albert Krastanov & Yousuke Kubota & Roumiana Tsenkova, 2015. "Monitoring of Water Spectral Pattern Reveals Differences in Probiotics Growth When Used for Rapid Bacteria Selection," PLOS ONE, Public Library of Science, vol. 10(7), pages 1-18, July.
    • Handle: RePEc:plo:pone00:0130698
    DOI: 10.1371/journal.pone.0130698
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    References listed on IDEAS

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    1. Joel G. Davis & Kamil P. Gierszal & Ping Wang & Dor Ben-Amotz, 2012. "Water structural transformation at molecular hydrophobic interfaces," Nature, Nature, vol. 491(7425), pages 582-585, November.
    2. Eri Chatani & Yutaro Tsuchisaka & Yuki Masuda & Roumiana Tsenkova, 2014. "Water Molecular System Dynamics Associated with Amyloidogenic Nucleation as Revealed by Real Time Near Infrared Spectroscopy and Aquaphotomics," PLOS ONE, Public Library of Science, vol. 9(7), pages 1-10, July.
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