IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0114844.html
   My bibliography  Save this article

A Novel Mini-DNA Barcoding Assay to Identify Processed Fins from Internationally Protected Shark Species

Author

Listed:
  • Andrew T Fields
  • Debra L Abercrombie
  • Rowena Eng
  • Kevin Feldheim
  • Demian D Chapman

Abstract

There is a growing need to identify shark products in trade, in part due to the recent listing of five commercially important species on the Appendices of the Convention on International Trade in Endangered Species (CITES; porbeagle, Lamna nasus, oceanic whitetip, Carcharhinus longimanus scalloped hammerhead, Sphyrna lewini, smooth hammerhead, S. zygaena and great hammerhead S. mokarran) in addition to three species listed in the early part of this century (whale, Rhincodon typus, basking, Cetorhinus maximus, and white, Carcharodon carcharias). Shark fins are traded internationally to supply the Asian dried seafood market, in which they are used to make the luxury dish shark fin soup. Shark fins usually enter international trade with their skin still intact and can be identified using morphological characters or standard DNA-barcoding approaches. Once they reach Asia and are traded in this region the skin is removed and they are treated with chemicals that eliminate many key diagnostic characters and degrade their DNA (“processed fins”). Here, we present a validated mini-barcode assay based on partial sequences of the cytochrome oxidase I gene that can reliably identify the processed fins of seven of the eight CITES listed shark species. We also demonstrate that the assay can even frequently identify the species or genus of origin of shark fin soup (31 out of 50 samples).

Suggested Citation

  • Andrew T Fields & Debra L Abercrombie & Rowena Eng & Kevin Feldheim & Demian D Chapman, 2015. "A Novel Mini-DNA Barcoding Assay to Identify Processed Fins from Internationally Protected Shark Species," PLOS ONE, Public Library of Science, vol. 10(2), pages 1-10, February.
    • Handle: RePEc:plo:pone00:0114844
    DOI: 10.1371/journal.pone.0114844
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0114844
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0114844&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0114844?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Worm, Boris & Davis, Brendal & Kettemer, Lisa & Ward-Paige, Christine A. & Chapman, Demian & Heithaus, Michael R. & Kessel, Steven T. & Gruber, Samuel H., 2013. "Global catches, exploitation rates, and rebuilding options for sharks," Marine Policy, Elsevier, vol. 40(C), pages 194-204.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Diego Cardeñosa & Andrew Fields & Debra Abercrombie & Kevin Feldheim & Stanley K H Shea & Demian D Chapman, 2017. "A multiplex PCR mini-barcode assay to identify processed shark products in the global trade," PLOS ONE, Public Library of Science, vol. 12(10), pages 1-9, October.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Erhardt, Tobias & Weder, Rolf, 2020. "Shark hunting: On the vulnerability of resources with heterogeneous species," Resource and Energy Economics, Elsevier, vol. 61(C).
    2. Sybersma, Stacie, 2015. "Review of shark legislation in Canada as a conservation tool," Marine Policy, Elsevier, vol. 61(C), pages 121-126.
    3. Humber, Frances & Andriamahefazafy, Mialy & Godley, Brendan John & Broderick, Annette Cameron, 2015. "Endangered, essential and exploited: How extant laws are not enough to protect marine megafauna in Madagascar," Marine Policy, Elsevier, vol. 60(C), pages 70-83.
    4. Friedrich, Laura A. & Jefferson, Rebecca & Glegg, Gillian, 2014. "Public perceptions of sharks: Gathering support for shark conservation," Marine Policy, Elsevier, vol. 47(C), pages 1-7.
    5. Diego Cardeñosa & Andrew Fields & Debra Abercrombie & Kevin Feldheim & Stanley K H Shea & Demian D Chapman, 2017. "A multiplex PCR mini-barcode assay to identify processed shark products in the global trade," PLOS ONE, Public Library of Science, vol. 12(10), pages 1-9, October.
    6. Oliver, Shelby & Braccini, Matias & Newman, Stephen J. & Harvey, Euan S., 2015. "Global patterns in the bycatch of sharks and rays," Marine Policy, Elsevier, vol. 54(C), pages 86-97.
    7. Erhardt, Tobias & Weder, Rolf, 2015. "Shark Hunting: International Trade and the Imminent Extinction of Heterogeneous Species," Working papers 2015/07, Faculty of Business and Economics - University of Basel.
    8. Youwei Xu & Xiaojie Dai & Zirong Huang & Mingshuai Sun & Zuozhi Chen & Kui Zhang, 2022. "Stock Assessment of Four Dominant Shark Bycatch Species in Bottom Trawl Fisheries in the Northern South China Sea," Sustainability, MDPI, vol. 14(7), pages 1-11, March.
    9. André S Afonso & Fábio H V Hazin, 2015. "Vertical Movement Patterns and Ontogenetic Niche Expansion in the Tiger Shark, Galeocerdo cuvier," PLOS ONE, Public Library of Science, vol. 10(1), pages 1-26, January.
    10. Ila France Porcher & Brian W. Darvell, 2022. "Shark Fishing vs. Conservation: Analysis and Synthesis," Sustainability, MDPI, vol. 14(15), pages 1-33, August.
    11. Rachel Bustamante, 2023. "Beyond Protection: Recognizing Nature’s Rights to Conserve Sharks," Sustainability, MDPI, vol. 15(9), pages 1-18, April.
    12. Po-Shun Chuang & Tzu-Chiao Hung & Hung-An Chang & Chien-Kang Huang & Jen-Chieh Shiao, 2016. "The Species and Origin of Shark Fins in Taiwan’s Fishing Ports, Markets, and Customs Detention: A DNA Barcoding Analysis," PLOS ONE, Public Library of Science, vol. 11(1), pages 1-13, January.
    13. Garla, Ricardo C. & Freitas, Renato H.A. & Calado, Janaina F. & Paterno, Gustavo B.C. & Carvalho, Adriana R., 2015. "Public awareness of the economic potential and threats to sharks of a tropical oceanic archipelago in the western South Atlantic," Marine Policy, Elsevier, vol. 60(C), pages 128-133.
    14. Mariana Tolotti & Patrice Guillotreau & Fabien Forget & Manuela Capello & Laurent Dagorn, 2023. "Unintended effects of single-species fisheries management," Environment, Development and Sustainability: A Multidisciplinary Approach to the Theory and Practice of Sustainable Development, Springer, vol. 25(9), pages 9227-9250, September.
    15. Manfredi Di Lorenzo & Antonio Calò & Antonio Di Franco & Giacomo Milisenda & Giorgio Aglieri & Carlo Cattano & Marco Milazzo & Paolo Guidetti, 2022. "Small-scale fisheries catch more threatened elasmobranchs inside partially protected areas than in unprotected areas," Nature Communications, Nature, vol. 13(1), pages 1-11, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0114844. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.