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Sheltering Behavior and Locomotor Activity in 11 Genetically Diverse Common Inbred Mouse Strains Using Home-Cage Monitoring

Author

Listed:
  • Maarten Loos
  • Bastijn Koopmans
  • Emmeke Aarts
  • Gregoire Maroteaux
  • Sophie van der Sluis
  • Neuro-BSIK Mouse Phenomics Consortium
  • Matthijs Verhage
  • August B Smit

Abstract

Functional genetic analyses in mice rely on efficient and in-depth characterization of the behavioral spectrum. Automated home-cage observation can provide a systematic and efficient screening method to detect unexplored, novel behavioral phenotypes. Here, we analyzed high-throughput automated home-cage data using existing and novel concepts, to detect a plethora of genetic differences in spontaneous behavior in a panel of commonly used inbred strains (129S1/SvImJ, A/J, C3H/HeJ, C57BL/6J, BALB/cJ, DBA/2J, NOD/LtJ, FVB/NJ, WSB/EiJ, PWK/PhJ and CAST/EiJ). Continuous video-tracking observations of sheltering behavior and locomotor activity were segmented into distinguishable behavioral elements, and studied at different time scales, yielding a set of 115 behavioral parameters of which 105 showed highly significant strain differences. This set of 115 parameters was highly dimensional; principal component analysis identified 26 orthogonal components with eigenvalues above one. Especially novel parameters of sheltering behavior and parameters describing aspects of motion of the mouse in the home-cage showed high genetic effect sizes. Multi-day habituation curves and patterns of behavior surrounding dark/light phase transitions showed striking strain differences, albeit with lower genetic effect sizes. This spontaneous home-cage behavior study demonstrates high dimensionality, with a strong genetic contribution to specific sets of behavioral measures. Importantly, spontaneous home-cage behavior analysis detects genetic effects that cannot be studied in conventional behavioral tests, showing that the inclusion of a few days of undisturbed, labor extensive home-cage assessment may greatly aid gene function analyses and drug target discovery.

Suggested Citation

  • Maarten Loos & Bastijn Koopmans & Emmeke Aarts & Gregoire Maroteaux & Sophie van der Sluis & Neuro-BSIK Mouse Phenomics Consortium & Matthijs Verhage & August B Smit, 2014. "Sheltering Behavior and Locomotor Activity in 11 Genetically Diverse Common Inbred Mouse Strains Using Home-Cage Monitoring," PLOS ONE, Public Library of Science, vol. 9(9), pages 1-9, September.
    • Handle: RePEc:plo:pone00:0108563
    DOI: 10.1371/journal.pone.0108563
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    1. Hueihan Jhuang & Estibaliz Garrote & Xinlin Yu & Vinita Khilnani & Tomaso Poggio & Andrew D. Steele & Thomas Serre, 2010. "Automated home-cage behavioural phenotyping of mice," Nature Communications, Nature, vol. 1(1), pages 1-10, December.
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