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Somatic Donor Cell Type Correlates with Embryonic, but Not Extra-Embryonic, Gene Expression in Postimplantation Cloned Embryos

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  • Ryutaro Hirasawa
  • Shogo Matoba
  • Kimiko Inoue
  • Atsuo Ogura

Abstract

The great majority of embryos generated by somatic cell nuclear transfer (SCNT) display defined abnormal phenotypes after implantation, such as an increased likelihood of death and abnormal placentation. To gain better insight into the underlying mechanisms, we analyzed genome-wide gene expression profiles of day 6.5 postimplantation mouse embryos cloned from three different cell types (cumulus cells, neonatal Sertoli cells and fibroblasts). The embryos retrieved from the uteri were separated into embryonic (epiblast) and extraembryonic (extraembryonic ectoderm and ectoplacental cone) tissues and were subjected to gene microarray analysis. Genotype- and sex-matched embryos produced by in vitro fertilization were used as controls. Principal component analysis revealed that whereas the gene expression patterns in the embryonic tissues varied according to the donor cell type, those in extraembryonic tissues were relatively consistent across all groups. Within each group, the embryonic tissues had more differentially expressed genes (DEGs) (>2-fold vs. controls) than did the extraembryonic tissues (P

Suggested Citation

  • Ryutaro Hirasawa & Shogo Matoba & Kimiko Inoue & Atsuo Ogura, 2013. "Somatic Donor Cell Type Correlates with Embryonic, but Not Extra-Embryonic, Gene Expression in Postimplantation Cloned Embryos," PLOS ONE, Public Library of Science, vol. 8(10), pages 1-11, October.
    • Handle: RePEc:plo:pone00:0076422
    DOI: 10.1371/journal.pone.0076422
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    1. Shinya Yamanaka & Helen M. Blau, 2010. "Nuclear reprogramming to a pluripotent state by three approaches," Nature, Nature, vol. 465(7299), pages 704-712, June.
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