IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0067411.html
   My bibliography  Save this article

Dynamic Activity of miR-125b and miR-93 during Murine Neural Stem Cell Differentiation In Vitro and in the Subventricular Zone Neurogenic Niche

Author

Listed:
  • Annalisa Lattanzi
  • Bernhard Gentner
  • Daniela Corno
  • Tiziano Di Tomaso
  • Pieter Mestdagh
  • Frank Speleman
  • Luigi Naldini
  • Angela Gritti

Abstract

Several microRNAs (miRNAs) that are either specifically enriched or highly expressed in neurons and glia have been described, but the identification of miRNAs modulating neural stem cell (NSC) biology remains elusive. In this study, we exploited high throughput miRNA expression profiling to identify candidate miRNAs enriched in NSC/early progenitors derived from the murine subventricular zone (SVZ). Then, we used lentiviral miRNA sensor vectors (LV.miRT) to monitor the activity of shortlisted miRNAs with cellular and temporal resolution during NSC differentiation, taking advantage of in vitro and in vivo models that recapitulate physiological neurogenesis and gliogenesis and using known neuronal- and glial-specific miRNAs as reference. The LV.miRT platform allowed us monitoring endogenous miRNA activity in low represented cell populations within a bulk culture or within the complexity of CNS tissue, with high sensitivity and specificity. In this way we validated and extended previous results on the neuronal-specific miR-124 and the astroglial-specific miR-23a. Importantly, we describe for the first time a cell type- and differentiation stage-specific modulation of miR-93 and miR-125b in SVZ-derived NSC cultures and in the SVZ neurogenic niche in vivo, suggesting key roles of these miRNAs in regulating NSC function.

Suggested Citation

  • Annalisa Lattanzi & Bernhard Gentner & Daniela Corno & Tiziano Di Tomaso & Pieter Mestdagh & Frank Speleman & Luigi Naldini & Angela Gritti, 2013. "Dynamic Activity of miR-125b and miR-93 during Murine Neural Stem Cell Differentiation In Vitro and in the Subventricular Zone Neurogenic Niche," PLOS ONE, Public Library of Science, vol. 8(6), pages 1-16, June.
    • Handle: RePEc:plo:pone00:0067411
    DOI: 10.1371/journal.pone.0067411
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0067411
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0067411&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0067411?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Lee P. Lim & Nelson C. Lau & Philip Garrett-Engele & Andrew Grimson & Janell M. Schelter & John Castle & David P. Bartel & Peter S. Linsley & Jason M. Johnson, 2005. "Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs," Nature, Nature, vol. 433(7027), pages 769-773, February.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Panagiotis Alexiou & Manolis Maragkakis & Giorgio L Papadopoulos & Victor A Simmosis & Lin Zhang & Artemis G Hatzigeorgiou, 2010. "The DIANA-mirExTra Web Server: From Gene Expression Data to MicroRNA Function," PLOS ONE, Public Library of Science, vol. 5(2), pages 1-7, February.
    2. Wenjie Zhu & Binghe Xu, 2014. "MicroRNA-21 Identified as Predictor of Cancer Outcome: A Meta-Analysis," PLOS ONE, Public Library of Science, vol. 9(8), pages 1-7, August.
    3. Weina Xu & Jinyi Liu & Huan Qi & Ruolin Si & Zhiguang Zhao & Zhiju Tao & Yuchuan Bai & Shipeng Hu & Xiaohan Sun & Yulin Cong & Haoye Zhang & Duchangjiang Fan & Long Xiao & Yangyang Wang & Yongbin Li &, 2024. "A lineage-resolved cartography of microRNA promoter activity in C. elegans empowers multidimensional developmental analysis," Nature Communications, Nature, vol. 15(1), pages 1-23, December.
    4. Ho Thi Bich Phuong & Vien Ngoc Thach & Luong Hoang Ngan & Le Thi Truc Linh, 2018. "Using Bioinformatics to predict potential targets of Microrna-144 in osteoarthritis," HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE - ENGINEERING AND TECHNOLOGY, HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE, HO CHI MINH CITY OPEN UNIVERSITY, vol. 8(1), pages 43-52.
    5. Rachel A Hillmer, 2015. "Systems Biology for Biologists," PLOS Pathogens, Public Library of Science, vol. 11(5), pages 1-6, May.
    6. Joel W Graff & Linda S Powers & Anne M Dickson & Jongkwang Kim & Anna C Reisetter & Ihab H Hassan & Karol Kremens & Thomas J Gross & Mary E Wilson & Martha M Monick, 2012. "Cigarette Smoking Decreases Global MicroRNA Expression in Human Alveolar Macrophages," PLOS ONE, Public Library of Science, vol. 7(8), pages 1-13, August.
    7. Urmo Võsa & Tõnu Esko & Silva Kasela & Tarmo Annilo, 2015. "Altered Gene Expression Associated with microRNA Binding Site Polymorphisms," PLOS ONE, Public Library of Science, vol. 10(10), pages 1-24, October.
    8. Ran Elkon & Reuven Agami, 2008. "Removal of AU Bias from Microarray mRNA Expression Data Enhances Computational Identification of Active MicroRNAs," PLOS Computational Biology, Public Library of Science, vol. 4(10), pages 1-10, October.
    9. Evelyn Zacharewicz & Paul Della Gatta & John Reynolds & Andrew Garnham & Tamsyn Crowley & Aaron P Russell & Séverine Lamon, 2014. "Identification of MicroRNAs Linked to Regulators of Muscle Protein Synthesis and Regeneration in Young and Old Skeletal Muscle," PLOS ONE, Public Library of Science, vol. 9(12), pages 1-25, December.
    10. Hotaka Kobayashi & Robert H. Singer, 2022. "Single-molecule imaging of microRNA-mediated gene silencing in cells," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0067411. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.