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qpure: A Tool to Estimate Tumor Cellularity from Genome-Wide Single-Nucleotide Polymorphism Profiles

Author

Listed:
  • Sarah Song
  • Katia Nones
  • David Miller
  • Ivon Harliwong
  • Karin S Kassahn
  • Mark Pinese
  • Marina Pajic
  • Anthony J Gill
  • Amber L Johns
  • Matthew Anderson
  • Oliver Holmes
  • Conrad Leonard
  • Darrin Taylor
  • Scott Wood
  • Qinying Xu
  • Felicity Newell
  • Mark J Cowley
  • Jianmin Wu
  • Peter Wilson
  • Lynn Fink
  • Andrew V Biankin
  • Nic Waddell
  • Sean M Grimmond
  • John V Pearson

Abstract

Tumour cellularity, the relative proportion of tumour and normal cells in a sample, affects the sensitivity of mutation detection, copy number analysis, cancer gene expression and methylation profiling. Tumour cellularity is traditionally estimated by pathological review of sectioned specimens; however this method is both subjective and prone to error due to heterogeneity within lesions and cellularity differences between the sample viewed during pathological review and tissue used for research purposes. In this paper we describe a statistical model to estimate tumour cellularity from SNP array profiles of paired tumour and normal samples using shifts in SNP allele frequency at regions of loss of heterozygosity (LOH) in the tumour. We also provide qpure, a software implementation of the method. Our experiments showed that there is a medium correlation 0.42 (-value = 0.0001) between tumor cellularity estimated by qpure and pathology review. Interestingly there is a high correlation 0.87 (-value 2.2e-16) between cellularity estimates by qpure and deep Ion Torrent sequencing of known somatic KRAS mutations; and a weaker correlation 0.32 (-value = 0.004) between IonTorrent sequencing and pathology review. This suggests that qpure may be a more accurate predictor of tumour cellularity than pathology review. qpure can be downloaded from https://sourceforge.net/projects/qpure/.

Suggested Citation

  • Sarah Song & Katia Nones & David Miller & Ivon Harliwong & Karin S Kassahn & Mark Pinese & Marina Pajic & Anthony J Gill & Amber L Johns & Matthew Anderson & Oliver Holmes & Conrad Leonard & Darrin Ta, 2012. "qpure: A Tool to Estimate Tumor Cellularity from Genome-Wide Single-Nucleotide Polymorphism Profiles," PLOS ONE, Public Library of Science, vol. 7(9), pages 1-7, September.
    • Handle: RePEc:plo:pone00:0045835
    DOI: 10.1371/journal.pone.0045835
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    Cited by:

    1. Marjan M. Naeini & Felicity Newell & Lauren G. Aoude & Vanessa F. Bonazzi & Kalpana Patel & Guy Lampe & Lambros T. Koufariotis & Vanessa Lakis & Venkateswar Addala & Olga Kondrashova & Rebecca L. John, 2023. "Multi-omic features of oesophageal adenocarcinoma in patients treated with preoperative neoadjuvant therapy," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    2. Julie Livingstone & Yu-Jia Shiah & Takafumi N. Yamaguchi & Lawrence E. Heisler & Vincent Huang & Robert Lesurf & Tsumugi Gebo & Benjamin Carlin & Stefan Eng & Erik Drysdale & Jeffrey Green & Theodorus, 2021. "The telomere length landscape of prostate cancer," Nature Communications, Nature, vol. 12(1), pages 1-13, December.

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