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Structures of the Signal Recognition Particle Receptor from the Archaeon Pyrococcus furiosus: Implications for the Targeting Step at the Membrane

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  • Pascal F Egea
  • Hiro Tsuruta
  • Gladys P de Leon
  • Johanna Napetschnig
  • Peter Walter
  • Robert M Stroud

Abstract

In all organisms, a ribonucleoprotein called the signal recognition particle (SRP) and its receptor (SR) target nascent proteins from the ribosome to the translocon for secretion or membrane insertion. We present the first X-ray structures of an archeal FtsY, the receptor from the hyper-thermophile Pyrococcus furiosus (Pfu), in its free and GDP•magnesium-bound forms. The highly charged N-terminal domain of Pfu-FtsY is distinguished by a long N-terminal helix. The basic charges on the surface of this helix are likely to regulate interactions at the membrane. A peripheral GDP bound near a regulatory motif could indicate a site of interaction between the receptor and ribosomal or SRP RNAs. Small angle X-ray scattering and analytical ultracentrifugation indicate that the crystal structure of Pfu-FtsY correlates well with the average conformation in solution. Based on previous structures of two sub-complexes, we propose a model of the core of archeal and eukaryotic SRP•SR targeting complexes.

Suggested Citation

  • Pascal F Egea & Hiro Tsuruta & Gladys P de Leon & Johanna Napetschnig & Peter Walter & Robert M Stroud, 2008. "Structures of the Signal Recognition Particle Receptor from the Archaeon Pyrococcus furiosus: Implications for the Targeting Step at the Membrane," PLOS ONE, Public Library of Science, vol. 3(11), pages 1-14, November.
    • Handle: RePEc:plo:pone00:0003619
    DOI: 10.1371/journal.pone.0003619
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    References listed on IDEAS

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    1. Pascal F. Egea & Shu-ou Shan & Johanna Napetschnig & David F. Savage & Peter Walter & Robert M. Stroud, 2004. "Substrate twinning activates the signal recognition particle and its receptor," Nature, Nature, vol. 427(6971), pages 215-221, January.
    2. Guillermo Montoya & Cecilia Svensson & Joen Luirink & Irmgard Sinning, 1997. "Crystal structure of the NG domain from the signal-recognition particle receptor FtsY," Nature, Nature, vol. 385(6614), pages 365-368, January.
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