Author
Listed:
- Nathaniel Burman
(Montana State University)
- Svetlana Belukhina
(The Center for Molecular and Cellular Biology)
- Florence Depardieu
(Institut Pasteur, Université Paris Cité, CNRS UMR 6047)
- Royce A. Wilkinson
(Montana State University)
- Mikhail Skutel
(The Center for Molecular and Cellular Biology)
- Andrew Santiago-Frangos
(Montana State University)
- Ava B. Graham
(Montana State University)
- Alexei Livenskyi
(Russian Academy of Sciences
Lomonosov Moscow State University)
- Anna Chechenina
(The Center for Molecular and Cellular Biology)
- Natalia Morozova
(Peter the Great St Petersburg State Polytechnic University)
- Trevor Zahl
(Montana State University)
- William S. Henriques
(Montana State University)
- Murat Buyukyoruk
(Montana State University)
- Christophe Rouillon
(Institut Pasteur, Université Paris Cité, CNRS UMR 6047)
- Baptiste Saudemont
(Institut Pasteur, Université Paris Cité, CNRS UMR 6047)
- Lena Shyrokova
(Lund University)
- Tatsuaki Kurata
(Lund University)
- Vasili Hauryliuk
(Lund University
Lund University
Science for Life Laboratory)
- Konstantin Severinov
(Russian Academy of Sciences
The State University of New Jersey)
- Justine Groseille
(Institut Pasteur, CNRS UMR 3525, Université Paris Cité
Université PSL
Sorbonne Université)
- Agnès Thierry
(Institut Pasteur, CNRS UMR 3525, Université Paris Cité)
- Romain Koszul
(Institut Pasteur, CNRS UMR 3525, Université Paris Cité)
- Florian Tesson
(Institut Pasteur, CNRS UMR3525, Université Paris Cité)
- Aude Bernheim
(Institut Pasteur, CNRS UMR3525, Université Paris Cité)
- David Bikard
(Institut Pasteur, Université Paris Cité, CNRS UMR 6047)
- Blake Wiedenheft
(Montana State University)
- Artem Isaev
(The Center for Molecular and Cellular Biology)
Abstract
Viruses compete with each other for limited cellular resources, and some deliver defence mechanisms that protect the host from competing genetic parasites1. The phage antirestriction induced system (PARIS) is a defence system, often encoded in viral genomes, that is composed of a 55 kDa ABC ATPase (AriA) and a 35 kDa TOPRIM nuclease (AriB)2. However, the mechanism by which AriA and AriB function in phage defence is unknown. Here we show that AriA and AriB assemble into a 425 kDa supramolecular immune complex. We use cryo-electron microscopy to determine the structure of this complex, thereby explaining how six molecules of AriA assemble into a propeller-shaped scaffold that coordinates three subunits of AriB. ATP-dependent detection of foreign proteins triggers the release of AriB, which assembles into a homodimeric nuclease that blocks infection by cleaving host lysine transfer RNA. Phage T5 subverts PARIS immunity through expression of a lysine transfer RNA variant that is not cleaved by PARIS, thereby restoring viral infection. Collectively, these data explain how AriA functions as an ATP-dependent sensor that detects viral proteins and activates the AriB toxin. PARIS is one of an emerging set of immune systems that form macromolecular complexes for the recognition of foreign proteins, rather than foreign nucleic acids3.
Suggested Citation
Nathaniel Burman & Svetlana Belukhina & Florence Depardieu & Royce A. Wilkinson & Mikhail Skutel & Andrew Santiago-Frangos & Ava B. Graham & Alexei Livenskyi & Anna Chechenina & Natalia Morozova & Tre, 2024.
"A virally encoded tRNA neutralizes the PARIS antiviral defence system,"
Nature, Nature, vol. 634(8033), pages 424-431, October.
Handle:
RePEc:nat:nature:v:634:y:2024:i:8033:d:10.1038_s41586-024-07874-3
DOI: 10.1038/s41586-024-07874-3
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