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Structural basis of gating modulation of Kv4 channel complexes

Author

Listed:
  • Yoshiaki Kise

    (The University of Tokyo)

  • Go Kasuya

    (Jichi Medical University)

  • Hiroyuki H. Okamoto

    (The University of Tokyo)

  • Daichi Yamanouchi

    (The University of Tokyo)

  • Kan Kobayashi

    (The University of Tokyo
    Peptidream)

  • Tsukasa Kusakizako

    (The University of Tokyo)

  • Tomohiro Nishizawa

    (The University of Tokyo
    Yokohama City University)

  • Koichi Nakajo

    (Jichi Medical University)

  • Osamu Nureki

    (The University of Tokyo)

Abstract

Modulation of voltage-gated potassium (Kv) channels by auxiliary subunits is central to the physiological function of channels in the brain and heart1,2. Native Kv4 tetrameric channels form macromolecular ternary complexes with two auxiliary β-subunits—intracellular Kv channel-interacting proteins (KChIPs) and transmembrane dipeptidyl peptidase-related proteins (DPPs)—to evoke rapidly activating and inactivating A-type currents, which prevent the backpropagation of action potentials1–5. However, the modulatory mechanisms of Kv4 channel complexes remain largely unknown. Here we report cryo-electron microscopy structures of the Kv4.2–DPP6S–KChIP1 dodecamer complex, the Kv4.2–KChIP1 and Kv4.2–DPP6S octamer complexes, and Kv4.2 alone. The structure of the Kv4.2–KChIP1 complex reveals that the intracellular N terminus of Kv4.2 interacts with its C terminus that extends from the S6 gating helix of the neighbouring Kv4.2 subunit. KChIP1 captures both the N and the C terminus of Kv4.2. In consequence, KChIP1 would prevent N-type inactivation and stabilize the S6 conformation to modulate gating of the S6 helices within the tetramer. By contrast, unlike the reported auxiliary subunits of voltage-gated channel complexes, DPP6S interacts with the S1 and S2 helices of the Kv4.2 voltage-sensing domain, which suggests that DPP6S stabilizes the conformation of the S1–S2 helices. DPP6S may therefore accelerate the voltage-dependent movement of the S4 helices. KChIP1 and DPP6S do not directly interact with each other in the Kv4.2–KChIP1–DPP6S ternary complex. Thus, our data suggest that two distinct modes of modulation contribute in an additive manner to evoke A-type currents from the native Kv4 macromolecular complex.

Suggested Citation

  • Yoshiaki Kise & Go Kasuya & Hiroyuki H. Okamoto & Daichi Yamanouchi & Kan Kobayashi & Tsukasa Kusakizako & Tomohiro Nishizawa & Koichi Nakajo & Osamu Nureki, 2021. "Structural basis of gating modulation of Kv4 channel complexes," Nature, Nature, vol. 599(7883), pages 158-164, November.
  • Handle: RePEc:nat:nature:v:599:y:2021:i:7883:d:10.1038_s41586-021-03935-z
    DOI: 10.1038/s41586-021-03935-z
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    Cited by:

    1. Qiansheng Liang & Gamma Chi & Leonardo Cirqueira & Lianteng Zhi & Agostino Marasco & Nadia Pilati & Martin J. Gunthorpe & Giuseppe Alvaro & Charles H. Large & David B. Sauer & Werner Treptow & Manuel , 2024. "The binding and mechanism of a positive allosteric modulator of Kv3 channels," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    2. Gamma Chi & Qiansheng Liang & Akshay Sridhar & John B. Cowgill & Kasim Sader & Mazdak Radjainia & Pu Qian & Pablo Castro-Hartmann & Shayla Venkaya & Nanki Kaur Singh & Gavin McKinley & Alejandra Ferna, 2022. "Cryo-EM structure of the human Kv3.1 channel reveals gating control by the cytoplasmic T1 domain," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    3. Martin J. Gunthorpe, 2022. "Timing is everything: structural insights into the disease-linked Kv3 channels controlling fast action-potential firing in the brain," Nature Communications, Nature, vol. 13(1), pages 1-4, December.
    4. Grace D. Galles & Daniel T. Infield & Colin J. Clark & Marcus L. Hemshorn & Shivani Manikandan & Frederico Fazan & Ali Rasouli & Emad Tajkhorshid & Jason D. Galpin & Richard B. Cooley & Ryan A. Mehl &, 2023. "Tuning phenylalanine fluorination to assess aromatic contributions to protein function and stability in cells," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

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