Author
Listed:
- Xiaodong Liu
(Monash University
Monash Biomedicine Discovery Institute
Monash University)
- Jia Ping Tan
(Monash University
Monash Biomedicine Discovery Institute
Monash University)
- Jan Schröder
(Monash University
Monash Biomedicine Discovery Institute
Monash University)
- Asma Aberkane
(Monash University)
- John F. Ouyang
(Duke-National University of Singapore Medical School)
- Monika Mohenska
(Monash University
Monash Biomedicine Discovery Institute
Monash University)
- Sue Mei Lim
(Monash University
Monash Biomedicine Discovery Institute
Monash University)
- Yu B. Y. Sun
(Monash University
Monash Biomedicine Discovery Institute
Monash University)
- Joseph Chen
(Monash University
Monash Biomedicine Discovery Institute
Monash University)
- Guizhi Sun
(Monash University
Monash Biomedicine Discovery Institute
Monash University)
- Yichen Zhou
(Monash University
Monash Biomedicine Discovery Institute
Monash University)
- Daniel Poppe
(The University of Western Australia
The Harry Perkins Institute of Medical Research)
- Ryan Lister
(The University of Western Australia
The Harry Perkins Institute of Medical Research)
- Amander T. Clark
(University of California Los Angeles
University of California Los Angeles
University of California Los Angeles
University of California Los Angeles)
- Owen J. L. Rackham
(Duke-National University of Singapore Medical School)
- Jennifer Zenker
(Monash University)
- Jose M. Polo
(Monash University
Monash Biomedicine Discovery Institute
Monash University)
Abstract
Human pluripotent and trophoblast stem cells have been essential alternatives to blastocysts for understanding early human development1–4. However, these simple culture systems lack the complexity to adequately model the spatiotemporal cellular and molecular dynamics that occur during early embryonic development. Here we describe the reprogramming of fibroblasts into in vitro three-dimensional models of the human blastocyst, termed iBlastoids. Characterization of iBlastoids shows that they model the overall architecture of blastocysts, presenting an inner cell mass-like structure, with epiblast- and primitive endoderm-like cells, a blastocoel-like cavity and a trophectoderm-like outer layer of cells. Single-cell transcriptomics further confirmed the presence of epiblast-, primitive endoderm-, and trophectoderm-like cells. Moreover, iBlastoids can give rise to pluripotent and trophoblast stem cells and are capable of modelling, in vitro, several aspects of the early stage of implantation. In summary, we have developed a scalable and tractable system to model human blastocyst biology; we envision that this will facilitate the study of early human development and the effects of gene mutations and toxins during early embryogenesis, as well as aiding in the development of new therapies associated with in vitro fertilization.
Suggested Citation
Xiaodong Liu & Jia Ping Tan & Jan Schröder & Asma Aberkane & John F. Ouyang & Monika Mohenska & Sue Mei Lim & Yu B. Y. Sun & Joseph Chen & Guizhi Sun & Yichen Zhou & Daniel Poppe & Ryan Lister & Amand, 2021.
"Modelling human blastocysts by reprogramming fibroblasts into iBlastoids,"
Nature, Nature, vol. 591(7851), pages 627-632, March.
Handle:
RePEc:nat:nature:v:591:y:2021:i:7851:d:10.1038_s41586-021-03372-y
DOI: 10.1038/s41586-021-03372-y
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Citations
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Cited by:
- Mingyue Guo & Jinyi Wu & Chuanxin Chen & Xinggu Wang & An Gong & Wei Guan & Rowan M. Karvas & Kexin Wang & Mingwei Min & Yixuan Wang & Thorold W. Theunissen & Shaorong Gao & José C. R. Silva, 2024.
"Self-renewing human naïve pluripotent stem cells dedifferentiate in 3D culture and form blastoids spontaneously,"
Nature Communications, Nature, vol. 15(1), pages 1-18, December.
- Denis Torre & Nancy J. Francoeur & Yael Kalma & Ilana Gross Carmel & Betsaida S. Melo & Gintaras Deikus & Kimaada Allette & Ron Flohr & Maya Fridrikh & Konstantinos Vlachos & Kent Madrid & Hardik Shah, 2023.
"Isoform-resolved transcriptome of the human preimplantation embryo,"
Nature Communications, Nature, vol. 14(1), pages 1-23, December.
- Yueli Yang & Wenqi Jia & Zhiwei Luo & Yunpan Li & Hao Liu & Lixin Fu & Jinxiu Li & Yu Jiang & Junjian Lai & Haiwei Li & Babangida Jabir Saeed & Yi Zou & Yuan Lv & Liang Wu & Ting Zhou & Yongli Shan & , 2024.
"VGLL1 cooperates with TEAD4 to control human trophectoderm lineage specification,"
Nature Communications, Nature, vol. 15(1), pages 1-18, December.
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