Author
Listed:
- Viridiana Olin-Sandoval
(University of Cambridge
Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán)
- Jason Shu Lim Yu
(The Francis Crick Institute)
- Leonor Miller-Fleming
(University of Cambridge
University of Cambridge)
- Mohammad Tauqeer Alam
(University of Warwick)
- Stephan Kamrad
(The Francis Crick Institute
University College London)
- Clara Correia-Melo
(The Francis Crick Institute)
- Robert Haas
(University of Cambridge
The Francis Crick Institute)
- Joanna Segal
(The Francis Crick Institute)
- David Alejandro Peña Navarro
(BOKU - University of Natural Resources and Life Sciences)
- Lucia Herrera-Dominguez
(The Francis Crick Institute)
- Oscar Méndez-Lucio
(Universidad Nacional Autónoma de México)
- Jakob Vowinckel
(University of Cambridge
Biognosys AG)
- Michael Mülleder
(University of Cambridge
The Francis Crick Institute
Charité University Medicine)
- Markus Ralser
(University of Cambridge
The Francis Crick Institute
Charité University Medicine)
Abstract
Both single and multicellular organisms depend on anti-stress mechanisms that enable them to deal with sudden changes in the environment, including exposure to heat and oxidants. Central to the stress response are dynamic changes in metabolism, such as the transition from the glycolysis to the pentose phosphate pathway—a conserved first-line response to oxidative insults1,2. Here we report a second metabolic adaptation that protects microbial cells in stress situations. The role of the yeast polyamine transporter Tpo1p3–5 in maintaining oxidant resistance is unknown6. However, a proteomic time-course experiment suggests a link to lysine metabolism. We reveal a connection between polyamine and lysine metabolism during stress situations, in the form of a promiscuous enzymatic reaction in which the first enzyme of the polyamine pathway, Spe1p, decarboxylates lysine and forms an alternative polyamine, cadaverine. The reaction proceeds in the presence of extracellular lysine, which is taken up by cells to reach concentrations up to one hundred times higher than those required for growth. Such extensive harvest is not observed for the other amino acids, is dependent on the polyamine pathway and triggers a reprogramming of redox metabolism. As a result, NADPH—which would otherwise be required for lysine biosynthesis—is channelled into glutathione metabolism, leading to a large increase in glutathione concentrations, lower levels of reactive oxygen species and increased oxidant tolerance. Our results show that nutrient uptake occurs not only to enable cell growth, but when the nutrient availability is favourable it also enables cells to reconfigure their metabolism to preventatively mount stress protection.
Suggested Citation
Viridiana Olin-Sandoval & Jason Shu Lim Yu & Leonor Miller-Fleming & Mohammad Tauqeer Alam & Stephan Kamrad & Clara Correia-Melo & Robert Haas & Joanna Segal & David Alejandro Peña Navarro & Lucia Her, 2019.
"Lysine harvesting is an antioxidant strategy and triggers underground polyamine metabolism,"
Nature, Nature, vol. 572(7768), pages 249-253, August.
Handle:
RePEc:nat:nature:v:572:y:2019:i:7768:d:10.1038_s41586-019-1442-6
DOI: 10.1038/s41586-019-1442-6
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Cited by:
- Giovanni Scarinci & Jan-Luca Ariens & Georgia Angelidou & Sebastian Schmidt & Timo Glatter & Nicole Paczia & Victor Sourjik, 2024.
"Enhanced metabolic entanglement emerges during the evolution of an interkingdom microbial community,"
Nature Communications, Nature, vol. 15(1), pages 1-13, December.
- Yuzhen Zhang & Yukmi Cai & Bing Zhang & Yi-Heng P. Job Zhang, 2024.
"Spatially structured exchange of metabolites enhances bacterial survival and resilience in biofilms,"
Nature Communications, Nature, vol. 15(1), pages 1-15, December.
- Ritu Gupta & Swagata Adhikary & Nidhi Dalpatraj & Sunil Laxman, 2024.
"An economic demand-based framework for prioritization strategies in response to transient amino acid limitations,"
Nature Communications, Nature, vol. 15(1), pages 1-12, December.
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