IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v565y2019i7739d10.1038_s41586-018-0847-y.html
   My bibliography  Save this article

Subcellular transcriptomes and proteomes of developing axon projections in the cerebral cortex

Author

Listed:
  • Alexandros Poulopoulos

    (Harvard University
    University of Maryland School of Medicine)

  • Alexander J. Murphy

    (Harvard University)

  • Abdulkadir Ozkan

    (Harvard University)

  • Patrick Davis

    (Harvard University)

  • John Hatch

    (Harvard University)

  • Rory Kirchner

    (Harvard University)

  • Jeffrey D. Macklis

    (Harvard University)

Abstract

The development of neural circuits relies on axon projections establishing diverse, yet well-defined, connections between areas of the nervous system. Each projection is formed by growth cones—subcellular specializations at the tips of growing axons, encompassing sets of molecules that control projection-specific growth, guidance, and target selection1. To investigate the set of molecules within native growth cones that form specific connections, here we developed growth cone sorting and subcellular RNA–proteome mapping, an approach that identifies and quantifies local transcriptomes and proteomes from labelled growth cones of single projections in vivo. Using this approach on the developing callosal projection of the mouse cerebral cortex, we mapped molecular enrichments in trans-hemispheric growth cones relative to their parent cell bodies, producing paired subcellular proteomes and transcriptomes from single neuron subtypes directly from the brain. These data provide generalizable proof-of-principle for this approach, and reveal molecular specializations of the growth cone, including accumulations of the growth-regulating kinase mTOR2, together with mRNAs that contain mTOR-dependent motifs3,4. These findings illuminate the relationships between subcellular distributions of RNA and protein in developing projection neurons, and provide a systems-level approach for the discovery of subtype- and stage-specific molecular substrates of circuit wiring, miswiring, and the potential for regeneration.

Suggested Citation

  • Alexandros Poulopoulos & Alexander J. Murphy & Abdulkadir Ozkan & Patrick Davis & John Hatch & Rory Kirchner & Jeffrey D. Macklis, 2019. "Subcellular transcriptomes and proteomes of developing axon projections in the cerebral cortex," Nature, Nature, vol. 565(7739), pages 356-360, January.
  • Handle: RePEc:nat:nature:v:565:y:2019:i:7739:d:10.1038_s41586-018-0847-y
    DOI: 10.1038/s41586-018-0847-y
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41586-018-0847-y
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.

    File URL: https://libkey.io/10.1038/s41586-018-0847-y?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Vincent Paget-Blanc & Marlene E. Pfeffer & Marie Pronot & Paul Lapios & Maria-Florencia Angelo & Roman Walle & Fabrice P. Cordelières & Florian Levet & Stéphane Claverol & Sabrina Lacomme & Mélina Pet, 2022. "A synaptomic analysis reveals dopamine hub synapses in the mouse striatum," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    2. Saeede Salehi & Abdolhossein Zare & Gianluca Prezza & Jakob Bader & Cornelius Schneider & Utz Fischer & Felix Meissner & Matthias Mann & Michael Briese & Michael Sendtner, 2023. "Cytosolic Ptbp2 modulates axon growth in motoneurons through axonal localization and translation of Hnrnpr," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    3. Claudia M. Fusco & Kristina Desch & Aline R. Dörrbaum & Mantian Wang & Anja Staab & Ivy C. W. Chan & Eleanor Vail & Veronica Villeri & Julian D. Langer & Erin M. Schuman, 2021. "Neuronal ribosomes exhibit dynamic and context-dependent exchange of ribosomal proteins," Nature Communications, Nature, vol. 12(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:565:y:2019:i:7739:d:10.1038_s41586-018-0847-y. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.