IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v563y2018i7732d10.1038_s41586-018-0714-x.html
   My bibliography  Save this article

POLAR-guided signalling complex assembly and localization drive asymmetric cell division

Author

Listed:
  • Anaxi Houbaert

    (Ghent University
    VIB)

  • Cheng Zhang

    (Ghent University
    VIB)

  • Manish Tiwari

    (Ghent University
    VIB)

  • Kun Wang

    (Ghent University
    VIB
    Wuhan University)

  • Alberto Marcos Serrano

    (Universidad de Castilla-la Mancha)

  • Daniel V. Savatin

    (Ghent University
    VIB)

  • Mounashree J. Urs

    (Ghent University
    VIB)

  • Miroslava K. Zhiponova

    (Ghent University
    VIB
    University of Sofia)

  • Gustavo E. Gudesblat

    (Ghent University
    VIB
    Universidad de Buenos Aires)

  • Isabelle Vanhoutte

    (Ghent University
    VIB)

  • Dominique Eeckhout

    (Ghent University
    VIB)

  • Sjef Boeren

    (Wageningen University)

  • Mansour Karimi

    (Ghent University
    VIB)

  • Camilla Betti

    (Ghent University
    VIB
    University of Perugia)

  • Thomas Jacobs

    (Ghent University
    VIB)

  • Carmen Fenoll

    (Universidad de Castilla-la Mancha)

  • Montaña Mena

    (Universidad de Castilla-la Mancha)

  • Sacco Vries

    (Wageningen University)

  • Geert Jaeger

    (Ghent University
    VIB)

  • Eugenia Russinova

    (Ghent University
    VIB)

Abstract

Stomatal cell lineage is an archetypal example of asymmetric cell division (ACD), which is necessary for plant survival1–4. In Arabidopsis thaliana, the GLYCOGEN SYNTHASE KINASE3 (GSK3)/SHAGGY-like kinase BRASSINOSTEROID INSENSITIVE 2 (BIN2) phosphorylates both the mitogen-activated protein kinase (MAPK) signalling module5,6 and its downstream target, the transcription factor SPEECHLESS (SPCH)7, to promote and restrict ACDs, respectively, in the same stomatal lineage cell. However, the mechanisms that balance these mutually exclusive activities remain unclear. Here we identify the plant-specific protein POLAR as a stomatal lineage scaffold for a subset of GSK3-like kinases that confines them to the cytosol and subsequently transiently polarizes them within the cell, together with BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE (BASL), before ACD. As a result, MAPK signalling is attenuated, enabling SPCH to drive ACD in the nucleus. Moreover, POLAR turnover requires phosphorylation on specific residues, mediated by GSK3. Our study reveals a mechanism by which the scaffolding protein POLAR ensures GSK3 substrate specificity, and could serve as a paradigm for understanding regulation of GSK3 in plants.

Suggested Citation

  • Anaxi Houbaert & Cheng Zhang & Manish Tiwari & Kun Wang & Alberto Marcos Serrano & Daniel V. Savatin & Mounashree J. Urs & Miroslava K. Zhiponova & Gustavo E. Gudesblat & Isabelle Vanhoutte & Dominiqu, 2018. "POLAR-guided signalling complex assembly and localization drive asymmetric cell division," Nature, Nature, vol. 563(7732), pages 574-578, November.
    • Handle: RePEc:nat:nature:v:563:y:2018:i:7732:d:10.1038_s41586-018-0714-x
    DOI: 10.1038/s41586-018-0714-x
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41586-018-0714-x
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/s41586-018-0714-x?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Ayami Nakagawa & Krishna Mohan Sepuru & Shu Jan Yip & Hyemin Seo & Calvin M. Coffin & Kota Hashimoto & Zixuan Li & Yasutomo Segawa & Rie Iwasaki & Hiroe Kato & Daisuke Kurihara & Yusuke Aihara & Steph, 2024. "Chemical inhibition of stomatal differentiation by perturbation of the master-regulatory bHLH heterodimer via an ACT-Like domain," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    2. Xiaoyu Guo & Xue Ding & Juan Dong, 2022. "Dichotomy of the BSL phosphatase signaling spatially regulates MAPK components in stomatal fate determination," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:563:y:2018:i:7732:d:10.1038_s41586-018-0714-x. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.