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POLAR-guided signalling complex assembly and localization drive asymmetric cell division

Author

Listed:
  • Anaxi Houbaert

    (Ghent University
    VIB)

  • Cheng Zhang

    (Ghent University
    VIB)

  • Manish Tiwari

    (Ghent University
    VIB)

  • Kun Wang

    (Ghent University
    VIB
    Wuhan University)

  • Alberto Marcos Serrano

    (Universidad de Castilla-la Mancha)

  • Daniel V. Savatin

    (Ghent University
    VIB)

  • Mounashree J. Urs

    (Ghent University
    VIB)

  • Miroslava K. Zhiponova

    (Ghent University
    VIB
    University of Sofia)

  • Gustavo E. Gudesblat

    (Ghent University
    VIB
    Universidad de Buenos Aires)

  • Isabelle Vanhoutte

    (Ghent University
    VIB)

  • Dominique Eeckhout

    (Ghent University
    VIB)

  • Sjef Boeren

    (Wageningen University)

  • Mansour Karimi

    (Ghent University
    VIB)

  • Camilla Betti

    (Ghent University
    VIB
    University of Perugia)

  • Thomas Jacobs

    (Ghent University
    VIB)

  • Carmen Fenoll

    (Universidad de Castilla-la Mancha)

  • Montaña Mena

    (Universidad de Castilla-la Mancha)

  • Sacco Vries

    (Wageningen University)

  • Geert Jaeger

    (Ghent University
    VIB)

  • Eugenia Russinova

    (Ghent University
    VIB)

Abstract

Stomatal cell lineage is an archetypal example of asymmetric cell division (ACD), which is necessary for plant survival1–4. In Arabidopsis thaliana, the GLYCOGEN SYNTHASE KINASE3 (GSK3)/SHAGGY-like kinase BRASSINOSTEROID INSENSITIVE 2 (BIN2) phosphorylates both the mitogen-activated protein kinase (MAPK) signalling module5,6 and its downstream target, the transcription factor SPEECHLESS (SPCH)7, to promote and restrict ACDs, respectively, in the same stomatal lineage cell. However, the mechanisms that balance these mutually exclusive activities remain unclear. Here we identify the plant-specific protein POLAR as a stomatal lineage scaffold for a subset of GSK3-like kinases that confines them to the cytosol and subsequently transiently polarizes them within the cell, together with BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE (BASL), before ACD. As a result, MAPK signalling is attenuated, enabling SPCH to drive ACD in the nucleus. Moreover, POLAR turnover requires phosphorylation on specific residues, mediated by GSK3. Our study reveals a mechanism by which the scaffolding protein POLAR ensures GSK3 substrate specificity, and could serve as a paradigm for understanding regulation of GSK3 in plants.

Suggested Citation

  • Anaxi Houbaert & Cheng Zhang & Manish Tiwari & Kun Wang & Alberto Marcos Serrano & Daniel V. Savatin & Mounashree J. Urs & Miroslava K. Zhiponova & Gustavo E. Gudesblat & Isabelle Vanhoutte & Dominiqu, 2018. "POLAR-guided signalling complex assembly and localization drive asymmetric cell division," Nature, Nature, vol. 563(7732), pages 574-578, November.
    • Handle: RePEc:nat:nature:v:563:y:2018:i:7732:d:10.1038_s41586-018-0714-x
    DOI: 10.1038/s41586-018-0714-x
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    Cited by:

    1. Xiaoyu Guo & Xue Ding & Juan Dong, 2022. "Dichotomy of the BSL phosphatase signaling spatially regulates MAPK components in stomatal fate determination," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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