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Visualizing late states of human 40S ribosomal subunit maturation

Author

Listed:
  • Michael Ameismeier

    (University of Munich)

  • Jingdong Cheng

    (University of Munich)

  • Otto Berninghausen

    (University of Munich)

  • Roland Beckmann

    (University of Munich)

Abstract

The formation of eukaryotic ribosomal subunits extends from the nucleolus to the cytoplasm and entails hundreds of assembly factors. Despite differences in the pathways of ribosome formation, high-resolution structural information has been available only from fungi. Here we present cryo-electron microscopy structures of late-stage human 40S assembly intermediates, representing one state reconstituted in vitro and five native states that range from nuclear to late cytoplasmic. The earliest particles reveal the position of the biogenesis factor RRP12 and distinct immature rRNA conformations that accompany the formation of the 40S subunit head. Molecular models of the late-acting assembly factors TSR1, RIOK1, RIOK2, ENP1, LTV1, PNO1 and NOB1 provide mechanistic details that underlie their contribution to a sequential 40S subunit assembly. The NOB1 architecture displays an inactive nuclease conformation that requires rearrangement of the PNO1-bound 3′ rRNA, thereby coordinating the final rRNA folding steps with site 3 cleavage.

Suggested Citation

  • Michael Ameismeier & Jingdong Cheng & Otto Berninghausen & Roland Beckmann, 2018. "Visualizing late states of human 40S ribosomal subunit maturation," Nature, Nature, vol. 558(7709), pages 249-253, June.
    • Handle: RePEc:nat:nature:v:558:y:2018:i:7709:d:10.1038_s41586-018-0193-0
    DOI: 10.1038/s41586-018-0193-0
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    Citations

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    Cited by:

    1. Ken Ikeuchi & Nives Ivic & Robert Buschauer & Jingdong Cheng & Thomas Fröhlich & Yoshitaka Matsuo & Otto Berninghausen & Toshifumi Inada & Thomas Becker & Roland Beckmann, 2023. "Molecular basis for recognition and deubiquitination of 40S ribosomes by Otu2," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    2. James C. Murphy & Elena M. Harrington & Sophie Schumann & Elton J. R. Vasconcelos & Timothy J. Mottram & Katherine L. Harper & Julie L. Aspden & Adrian Whitehouse, 2023. "Kaposi’s sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    3. Shrestha Ghosh & Mileena T. Nguyen & Ha Eun Choi & Maximilian Stahl & Annemarie Luise Kühn & Sandra Auwera & Hans J. Grabe & Henry Völzke & Georg Homuth & Samuel A. Myers & Cory M. Hogaboam & Imre Not, 2024. "RIOK2 transcriptionally regulates TRiC and dyskerin complexes to prevent telomere shortening," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    4. Xiaomeng Liang & Mei-Qing Zuo & Yunyang Zhang & Ningning Li & Chengying Ma & Meng-Qiu Dong & Ning Gao, 2020. "Structural snapshots of human pre-60S ribosomal particles before and after nuclear export," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    5. Lisa Kofler & Lorenz Grundmann & Magdalena Gerhalter & Michael Prattes & Juliane Merl-Pham & Gertrude Zisser & Irina Grishkovskaya & Victor-Valentin Hodirnau & Martin Vareka & Rolf Breinbauer & Stefan, 2024. "The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

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