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Structure of the peptidoglycan polymerase RodA resolved by evolutionary coupling analysis

Author

Listed:
  • Megan Sjodt

    (Harvard Medical School)

  • Kelly Brock

    (Harvard Medical School)

  • Genevieve Dobihal

    (Harvard Medical School)

  • Patricia D. A. Rohs

    (Harvard Medical School)

  • Anna G. Green

    (Harvard Medical School)

  • Thomas A. Hopf

    (Harvard Medical School)

  • Alexander J. Meeske

    (Harvard Medical School)

  • Veerasak Srisuknimit

    (Harvard University)

  • Daniel Kahne

    (Harvard University)

  • Suzanne Walker

    (Harvard Medical School)

  • Debora S. Marks

    (Harvard Medical School)

  • Thomas G. Bernhardt

    (Harvard Medical School)

  • David Z. Rudner

    (Harvard Medical School)

  • Andrew C. Kruse

    (Harvard Medical School)

Abstract

Evolutionary coupling-enabled molecular replacement determination of the structure of Thermus thermophilus RodA reveals a highly conserved cavity in its transmembrane domain, and mutagenesis experiments in Bacillus subtilis and Escherichia coli show that perturbation of this cavity abolishes RodA function.

Suggested Citation

  • Megan Sjodt & Kelly Brock & Genevieve Dobihal & Patricia D. A. Rohs & Anna G. Green & Thomas A. Hopf & Alexander J. Meeske & Veerasak Srisuknimit & Daniel Kahne & Suzanne Walker & Debora S. Marks & Th, 2018. "Structure of the peptidoglycan polymerase RodA resolved by evolutionary coupling analysis," Nature, Nature, vol. 556(7699), pages 118-121, April.
    • Handle: RePEc:nat:nature:v:556:y:2018:i:7699:d:10.1038_nature25985
    DOI: 10.1038/nature25985
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    Cited by:

    1. Irina Shlosman & Elayne M. Fivenson & Morgan S. A. Gilman & Tyler A. Sisley & Suzanne Walker & Thomas G. Bernhardt & Andrew C. Kruse & Joseph J. Loparo, 2023. "Allosteric activation of cell wall synthesis during bacterial growth," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Brooke M. Britton & Remy A. Yovanno & Sara F. Costa & Joshua McCausland & Albert Y. Lau & Jie Xiao & Zach Hensel, 2023. "Conformational changes in the essential E. coli septal cell wall synthesis complex suggest an activation mechanism," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    3. Rie Nygaard & Chris L. B. Graham & Meagan Belcher Dufrisne & Jonathan D. Colburn & Joseph Pepe & Molly A. Hydorn & Silvia Corradi & Chelsea M. Brown & Khuram U. Ashraf & Owen N. Vickery & Nicholas S. , 2023. "Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

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