IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v549y2017i7670d10.1038_nature23875.html
   My bibliography  Save this article

Discovery of stimulation-responsive immune enhancers with CRISPR activation

Author

Listed:
  • Dimitre R. Simeonov

    (Biomedical Sciences Graduate Program, University of California
    University of California
    Diabetes Center, University of California
    Innovative Genomics Institute, University of California)

  • Benjamin G. Gowen

    (Innovative Genomics Institute, University of California
    University of California, Berkeley)

  • Mandy Boontanrart

    (Innovative Genomics Institute, University of California
    University of California, Berkeley)

  • Theodore L. Roth

    (Biomedical Sciences Graduate Program, University of California
    University of California
    Diabetes Center, University of California
    Innovative Genomics Institute, University of California)

  • John D. Gagnon

    (Biomedical Sciences Graduate Program, University of California
    University of California
    Sandler Asthma Basic Research Center, University of California)

  • Maxwell R. Mumbach

    (Center for Personal Dynamic Regulomes, Stanford University School of Medicine
    Program in Epithelial Biology, Stanford University School of Medicine
    Stanford University School of Medicine)

  • Ansuman T. Satpathy

    (Center for Personal Dynamic Regulomes, Stanford University School of Medicine
    Stanford University School of Medicine)

  • Youjin Lee

    (University of California
    Diabetes Center, University of California
    Innovative Genomics Institute, University of California)

  • Nicolas L. Bray

    (Innovative Genomics Institute, University of California
    University of California, Berkeley)

  • Alice Y. Chan

    (Diabetes Center, University of California
    University of California)

  • Dmytro S. Lituiev

    (Institute for Human Genetics (IHG), University of California)

  • Michelle L. Nguyen

    (University of California
    Diabetes Center, University of California
    Innovative Genomics Institute, University of California)

  • Rachel E. Gate

    (Institute for Human Genetics (IHG), University of California
    Biological and Medical Informatics Graduate Program, University of California)

  • Meena Subramaniam

    (Institute for Human Genetics (IHG), University of California
    Biological and Medical Informatics Graduate Program, University of California)

  • Zhongmei Li

    (University of California
    Diabetes Center, University of California
    Innovative Genomics Institute, University of California)

  • Jonathan M. Woo

    (University of California
    Diabetes Center, University of California
    Innovative Genomics Institute, University of California)

  • Therese Mitros

    (Innovative Genomics Institute, University of California
    University of California, Berkeley)

  • Graham J. Ray

    (Innovative Genomics Institute, University of California
    University of California, Berkeley)

  • Gemma L. Curie

    (Innovative Genomics Institute, University of California
    University of California, Berkeley)

  • Nicki Naddaf

    (Innovative Genomics Institute, University of California
    University of California, Berkeley)

  • Julia S. Chu

    (Innovative Genomics Institute, University of California
    University of California, Berkeley)

  • Hong Ma

    (Innovative Genomics Institute, University of California
    University of California, Berkeley)

  • Eric Boyer

    (Diabetes Center, University of California
    Innovative Genomics Institute, University of California)

  • Frederic Van Gool

    (Diabetes Center, University of California)

  • Hailiang Huang

    (Broad Institute of MIT and Harvard
    Analytic and Translational Genetics Unit, Massachusetts General Hospital, Harvard Medical School)

  • Ruize Liu

    (Broad Institute of MIT and Harvard
    Analytic and Translational Genetics Unit, Massachusetts General Hospital, Harvard Medical School)

  • Victoria R. Tobin

    (University of California
    Diabetes Center, University of California
    Innovative Genomics Institute, University of California)

  • Kathrin Schumann

    (University of California
    Diabetes Center, University of California
    Innovative Genomics Institute, University of California)

  • Mark J. Daly

    (Broad Institute of MIT and Harvard
    Analytic and Translational Genetics Unit, Massachusetts General Hospital, Harvard Medical School)

  • Kyle K. Farh

    (Illumina Inc., 5200 Illumina Way)

  • K. Mark Ansel

    (University of California
    Sandler Asthma Basic Research Center, University of California)

  • Chun J. Ye

    (Institute for Human Genetics (IHG), University of California)

  • William J. Greenleaf

    (Center for Personal Dynamic Regulomes, Stanford University School of Medicine
    Stanford University School of Medicine
    Stanford University
    Chan Zuckerberg Biohub)

  • Mark S. Anderson

    (Diabetes Center, University of California
    University of California)

  • Jeffrey A. Bluestone

    (Diabetes Center, University of California)

  • Howard Y. Chang

    (Center for Personal Dynamic Regulomes, Stanford University School of Medicine
    Program in Epithelial Biology, Stanford University School of Medicine)

  • Jacob E. Corn

    (Innovative Genomics Institute, University of California
    University of California, Berkeley)

  • Alexander Marson

    (University of California
    Diabetes Center, University of California
    Innovative Genomics Institute, University of California
    Chan Zuckerberg Biohub)

Abstract

The authors use tiled CRISPR activation for functional enhancer discovery across two autoimmunity risk loci, CD69 and IL2RA, and identify elements with features of stimulus-responsive enhancers, including an IL2RA enhancer that harbours a fine-mapped autoimmunity risk variant.

Suggested Citation

  • Dimitre R. Simeonov & Benjamin G. Gowen & Mandy Boontanrart & Theodore L. Roth & John D. Gagnon & Maxwell R. Mumbach & Ansuman T. Satpathy & Youjin Lee & Nicolas L. Bray & Alice Y. Chan & Dmytro S. Li, 2017. "Discovery of stimulation-responsive immune enhancers with CRISPR activation," Nature, Nature, vol. 549(7670), pages 111-115, September.
    • Handle: RePEc:nat:nature:v:549:y:2017:i:7670:d:10.1038_nature23875
    DOI: 10.1038/nature23875
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature23875
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature23875?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Tian Zhou & Xinyi Zhu & Zhizhong Ye & Yong-Fei Wang & Chao Yao & Ning Xu & Mi Zhou & Jianyang Ma & Yuting Qin & Yiwei Shen & Yuanjia Tang & Zhihua Yin & Hong Xu & Yutong Zhang & Xiaoli Zang & Huihua D, 2022. "Lupus enhancer risk variant causes dysregulation of IRF8 through cooperative lncRNA and DNA methylation machinery," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    2. B. Edginton-White & A. Maytum & S. G. Kellaway & D. K. Goode & P. Keane & I. Pagnuco & S. A. Assi & L. Ames & M. Clarke & P. N. Cockerill & B. Göttgens & J. B. Cazier & C. Bonifer, 2023. "A genome-wide relay of signalling-responsive enhancers drives hematopoietic specification," Nature Communications, Nature, vol. 14(1), pages 1-20, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:549:y:2017:i:7670:d:10.1038_nature23875. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.