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Applying systems-level spectral imaging and analysis to reveal the organelle interactome

Author

Listed:
  • Alex M. Valm

    (Eunice Kennedy Shriver National Institute for Child Health and Human Development, NIH)

  • Sarah Cohen

    (Eunice Kennedy Shriver National Institute for Child Health and Human Development, NIH)

  • Wesley R. Legant

    (Janelia Research Campus, Howard Hughes Medical Institute)

  • Justin Melunis

    (School of Biomedical Engineering, Science and Health Systems, Drexel University)

  • Uri Hershberg

    (School of Biomedical Engineering, Science and Health Systems, Drexel University
    College of Medicine, Drexel University)

  • Eric Wait

    (College of Engineering, Drexel University)

  • Andrew R. Cohen

    (College of Engineering, Drexel University)

  • Michael W. Davidson

    (The Florida State University)

  • Eric Betzig

    (Janelia Research Campus, Howard Hughes Medical Institute)

  • Jennifer Lippincott-Schwartz

    (Eunice Kennedy Shriver National Institute for Child Health and Human Development, NIH
    Janelia Research Campus, Howard Hughes Medical Institute)

Abstract

Using confocal and lattice light sheet microscopy, the authors perform systems-level analysis of the organelle interactome in live cells, allowing them to visualize the frequency and locality of up to five-way interactions between different organelles.

Suggested Citation

  • Alex M. Valm & Sarah Cohen & Wesley R. Legant & Justin Melunis & Uri Hershberg & Eric Wait & Andrew R. Cohen & Michael W. Davidson & Eric Betzig & Jennifer Lippincott-Schwartz, 2017. "Applying systems-level spectral imaging and analysis to reveal the organelle interactome," Nature, Nature, vol. 546(7656), pages 162-167, June.
    • Handle: RePEc:nat:nature:v:546:y:2017:i:7656:d:10.1038_nature22369
    DOI: 10.1038/nature22369
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    Cited by:

    1. Katelyn C. Cook & Elene Tsopurashvili & Jason M. Needham & Sunnie R. Thompson & Ileana M. Cristea, 2022. "Restructured membrane contacts rewire organelles for human cytomegalovirus infection," Nature Communications, Nature, vol. 13(1), pages 1-20, December.
    2. Noble Kumar Talari & Ushodaya Mattam & Niroj Kumar Meher & Arun Kumar Paripati & Kalyankar Mahadev & Thanuja Krishnamoorthy & Naresh Babu V. Sepuri, 2023. "Lipid-droplet associated mitochondria promote fatty-acid oxidation through a distinct bioenergetic pattern in male Wistar rats," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    3. Dianne Lumaquin-Yin & Emily Montal & Eleanor Johns & Arianna Baggiolini & Ting-Hsiang Huang & Yilun Ma & Charlotte LaPlante & Shruthy Suresh & Lorenz Studer & Richard M. White, 2023. "Lipid droplets are a metabolic vulnerability in melanoma," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    4. Jan Kretschmer & Tomáš David & Martin Dračínský & Ondřej Socha & Daniel Jirak & Martin Vít & Radek Jurok & Martin Kuchař & Ivana Císařová & Miloslav Polasek, 2022. "Paramagnetic encoding of molecules," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    5. Yong Mi Choi & Dalila Ajjaji & Kaelin D. Fleming & Peter P. Borbat & Meredith L. Jenkins & Brandon E. Moeller & Shaveen Fernando & Surita R. Bhatia & Jack H. Freed & John E. Burke & Abdou Rachid Thiam, 2023. "Structural insights into perilipin 3 membrane association in response to diacylglycerol accumulation," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    6. Archibald Enninful & Alev Baysoy & Rong Fan, 2022. "Unmixing for ultra-high-plex fluorescence imaging," Nature Communications, Nature, vol. 13(1), pages 1-3, December.
    7. Raja Chouket & Agnès Pellissier-Tanon & Aliénor Lahlou & Ruikang Zhang & Diana Kim & Marie-Aude Plamont & Mingshu Zhang & Xi Zhang & Pingyong Xu & Nicolas Desprat & Dominique Bourgeois & Agathe Espagn, 2022. "Extra kinetic dimensions for label discrimination," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    8. Güneş Parlakgül & Song Pang & Leonardo L. Artico & Nina Min & Erika Cagampan & Reyna Villa & Renata L. S. Goncalves & Grace Yankun Lee & C. Shan Xu & Gökhan S. Hotamışlıgil & Ana Paula Arruda, 2024. "Spatial mapping of hepatic ER and mitochondria architecture reveals zonated remodeling in fasting and obesity," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    9. Cátia Silva Janota & Andreia Pinto & Anna Pezzarossa & Pedro Machado & Judite Costa & Pedro Campinho & Cláudio A. Franco & Edgar R. Gomes, 2022. "Shielding of actin by the endoplasmic reticulum impacts nuclear positioning," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    10. Alexandre Santinho & Maxime Carpentier & Julio Lopes Sampaio & Mohyeddine Omrane & Abdou Rachid Thiam, 2024. "Giant organelle vesicles to uncover intracellular membrane mechanics and plasticity," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    11. Junyoung Seo & Yeonbo Sim & Jeewon Kim & Hyunwoo Kim & In Cho & Hoyeon Nam & Young-Gyu Yoon & Jae-Byum Chang, 2022. "PICASSO allows ultra-multiplexed fluorescence imaging of spatially overlapping proteins without reference spectra measurements," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    12. James Burgess & Jeffrey J. Nirschl & Maria-Clara Zanellati & Alejandro Lozano & Sarah Cohen & Serena Yeung-Levy, 2024. "Orientation-invariant autoencoders learn robust representations for shape profiling of cells and organelles," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

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