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Applying systems-level spectral imaging and analysis to reveal the organelle interactome

Author

Listed:
  • Alex M. Valm

    (Eunice Kennedy Shriver National Institute for Child Health and Human Development, NIH)

  • Sarah Cohen

    (Eunice Kennedy Shriver National Institute for Child Health and Human Development, NIH)

  • Wesley R. Legant

    (Janelia Research Campus, Howard Hughes Medical Institute)

  • Justin Melunis

    (School of Biomedical Engineering, Science and Health Systems, Drexel University)

  • Uri Hershberg

    (School of Biomedical Engineering, Science and Health Systems, Drexel University
    College of Medicine, Drexel University)

  • Eric Wait

    (College of Engineering, Drexel University)

  • Andrew R. Cohen

    (College of Engineering, Drexel University)

  • Michael W. Davidson

    (The Florida State University)

  • Eric Betzig

    (Janelia Research Campus, Howard Hughes Medical Institute)

  • Jennifer Lippincott-Schwartz

    (Eunice Kennedy Shriver National Institute for Child Health and Human Development, NIH
    Janelia Research Campus, Howard Hughes Medical Institute)

Abstract

Using confocal and lattice light sheet microscopy, the authors perform systems-level analysis of the organelle interactome in live cells, allowing them to visualize the frequency and locality of up to five-way interactions between different organelles.

Suggested Citation

  • Alex M. Valm & Sarah Cohen & Wesley R. Legant & Justin Melunis & Uri Hershberg & Eric Wait & Andrew R. Cohen & Michael W. Davidson & Eric Betzig & Jennifer Lippincott-Schwartz, 2017. "Applying systems-level spectral imaging and analysis to reveal the organelle interactome," Nature, Nature, vol. 546(7656), pages 162-167, June.
    • Handle: RePEc:nat:nature:v:546:y:2017:i:7656:d:10.1038_nature22369
    DOI: 10.1038/nature22369
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    15. Junyoung Seo & Yeonbo Sim & Jeewon Kim & Hyunwoo Kim & In Cho & Hoyeon Nam & Young-Gyu Yoon & Jae-Byum Chang, 2022. "PICASSO allows ultra-multiplexed fluorescence imaging of spatially overlapping proteins without reference spectra measurements," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
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