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Regulation of RNA polymerase II activation by histone acetylation in single living cells

Author

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  • Timothy J. Stasevich

    (Graduate School of Frontier Biosciences, Osaka University, Osaka, 565-0871, Japan
    Colorado State University
    Transcription Imaging Consortium, Janelia Farm Research Campus, Howard Hughes Medical Institute)

  • Yoko Hayashi-Takanaka

    (Graduate School of Frontier Biosciences, Osaka University, Osaka, 565-0871, Japan
    Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Saitama, 332-0012, Japan
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, 226-8501, Japan)

  • Yuko Sato

    (Graduate School of Frontier Biosciences, Osaka University, Osaka, 565-0871, Japan
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, 226-8501, Japan)

  • Kazumitsu Maehara

    (Faculty of Medicine, Kyushu University, Fukuoka, 812-8582, Japan)

  • Yasuyuki Ohkawa

    (Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Saitama, 332-0012, Japan
    Faculty of Medicine, Kyushu University, Fukuoka, 812-8582, Japan)

  • Kumiko Sakata-Sogawa

    (Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, 226-8501, Japan
    RIKEN Center for Integrative Medical Sciences (IMS), Yokohama, 230-0045, Japan)

  • Makio Tokunaga

    (Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, 226-8501, Japan
    RIKEN Center for Integrative Medical Sciences (IMS), Yokohama, 230-0045, Japan)

  • Takahiro Nagase

    (Kazusa DNA Research Institute, Chiba, 292-0818, Japan)

  • Naohito Nozaki

    (Mab Institute Inc., Sapporo, 001-0021, Japan)

  • James G. McNally

    (Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health
    Institute for Soft Matter and Functional Materials, Helmholtz Zentrum Berlin, Berlin, 14109, Germany)

  • Hiroshi Kimura

    (Graduate School of Frontier Biosciences, Osaka University, Osaka, 565-0871, Japan
    Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Saitama, 332-0012, Japan
    Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, 226-8501, Japan)

Abstract

The interplay of histone acetylation and RNA polymerase II activity is investigated using fluorescence microscopy; acetylation of H3 at Lys 27 enhances the recruitment of a transcriptional activator and accelerates the transition of RNA polymerase II from initiation to elongation, thus indicating that histone acetylation has a causal effect on two distinct steps in transcription activation.

Suggested Citation

  • Timothy J. Stasevich & Yoko Hayashi-Takanaka & Yuko Sato & Kazumitsu Maehara & Yasuyuki Ohkawa & Kumiko Sakata-Sogawa & Makio Tokunaga & Takahiro Nagase & Naohito Nozaki & James G. McNally & Hiroshi K, 2014. "Regulation of RNA polymerase II activation by histone acetylation in single living cells," Nature, Nature, vol. 516(7530), pages 272-275, December.
    • Handle: RePEc:nat:nature:v:516:y:2014:i:7530:d:10.1038_nature13714
    DOI: 10.1038/nature13714
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    Citations

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    Cited by:

    1. Kosuke Tomimatsu & Takeru Fujii & Ryoma Bise & Kazufumi Hosoda & Yosuke Taniguchi & Hiroshi Ochiai & Hiroaki Ohishi & Kanta Ando & Ryoma Minami & Kaori Tanaka & Taro Tachibana & Seiichi Mori & Akihito, 2024. "Precise immunofluorescence canceling for highly multiplexed imaging to capture specific cell states," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    2. Ryuichiro Nakato & Toyonori Sakata & Jiankang Wang & Luis Augusto Eijy Nagai & Yuya Nagaoka & Gina Miku Oba & Masashige Bando & Katsuhiko Shirahige, 2023. "Context-dependent perturbations in chromatin folding and the transcriptome by cohesin and related factors," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    3. Hiroaki Ohishi & Seiru Shimada & Satoshi Uchino & Jieru Li & Yuko Sato & Manabu Shintani & Hitoshi Owada & Yasuyuki Ohkawa & Alexandros Pertsinidis & Takashi Yamamoto & Hiroshi Kimura & Hiroshi Ochiai, 2022. "STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    4. Jiankang Wang & Masashige Bando & Katsuhiko Shirahige & Ryuichiro Nakato, 2022. "Large-scale multi-omics analysis suggests specific roles for intragenic cohesin in transcriptional regulation," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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