IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v510y2014i7504d10.1038_nature13234.html
   My bibliography  Save this article

A Ctf4 trimer couples the CMG helicase to DNA polymerase α in the eukaryotic replisome

Author

Listed:
  • Aline C. Simon

    (University of Cambridge, Cambridge CB2 1GA, UK)

  • Jin C. Zhou

    (Clare Hall Laboratories, Cancer Research UK London Research Institute, London EN6 3LD, UK)

  • Rajika L. Perera

    (University of Cambridge, Cambridge CB2 1GA, UK
    Present addresses: Imperial College, South Kensington, London SW7 2AZ, UK (R.L.P.); Cancer Research UK London Research Institute, London WC2A 3LY, UK (M.E.I.).)

  • Frederick van Deursen

    (Cancer Research UK Manchester Institute, University of Manchester, Manchester M20 4BX, UK)

  • Cecile Evrin

    (MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK)

  • Marina E. Ivanova

    (University of Cambridge, Cambridge CB2 1GA, UK
    Present addresses: Imperial College, South Kensington, London SW7 2AZ, UK (R.L.P.); Cancer Research UK London Research Institute, London WC2A 3LY, UK (M.E.I.).)

  • Mairi L. Kilkenny

    (University of Cambridge, Cambridge CB2 1GA, UK)

  • Ludovic Renault

    (Clare Hall Laboratories, Cancer Research UK London Research Institute, London EN6 3LD, UK)

  • Svend Kjaer

    (Protein purification, Cancer Research UK London Research Institute, London WC2A 3LY, UK)

  • Dijana Matak-Vinković

    (University of Cambridge, Cambridge CB2 1EW, UK)

  • Karim Labib

    (MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, Dundee DD1 5EH, UK)

  • Alessandro Costa

    (Clare Hall Laboratories, Cancer Research UK London Research Institute, London EN6 3LD, UK)

  • Luca Pellegrini

    (University of Cambridge, Cambridge CB2 1GA, UK)

Abstract

This study shows how the yeast Ctf4 protein couples the DNA helicase, Cdc45–MCM–GINS, to DNA polymerase α — the GINS subunit of the helicase and the polymerase use a similar interaction to bind Ctf4, suggesting that, as Ctf4 is a trimer, two polymerases could be simultaneously coupled to a single helicase during lagging-strand synthesis.

Suggested Citation

  • Aline C. Simon & Jin C. Zhou & Rajika L. Perera & Frederick van Deursen & Cecile Evrin & Marina E. Ivanova & Mairi L. Kilkenny & Ludovic Renault & Svend Kjaer & Dijana Matak-Vinković & Karim Labib & A, 2014. "A Ctf4 trimer couples the CMG helicase to DNA polymerase α in the eukaryotic replisome," Nature, Nature, vol. 510(7504), pages 293-297, June.
    • Handle: RePEc:nat:nature:v:510:y:2014:i:7504:d:10.1038_nature13234
    DOI: 10.1038/nature13234
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature13234
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature13234?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Chiara Masnovo & Zohar Paleiov & Daniel Dovrat & Laurel K. Baxter & Sofia Movafaghi & Amir Aharoni & Sergei M. Mirkin, 2024. "Stabilization of expandable DNA repeats by the replication factor Mcm10 promotes cell viability," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:510:y:2014:i:7504:d:10.1038_nature13234. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.