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A map of nucleosome positions in yeast at base-pair resolution

Author

Listed:
  • Kristin Brogaard

    (Northwestern University)

  • Liqun Xi

    (Northwestern University)

  • Ji-Ping Wang

    (Northwestern University)

  • Jonathan Widom

    (Northwestern University
    Northwestern University)

Abstract

The exact positions of nucleosomes along genomic DNA can influence many aspects of chromosome function. However, existing methods for mapping nucleosomes do not provide the necessary single-base-pair accuracy to determine these positions. Here we develop and apply a new approach for direct mapping of nucleosome centres on the basis of chemical modification of engineered histones. The resulting map locates nucleosome positions genome-wide in unprecedented detail and accuracy. It shows new aspects of the in vivo nucleosome organization that are linked to transcription factor binding, RNA polymerase pausing and the higher-order structure of the chromatin fibre.

Suggested Citation

  • Kristin Brogaard & Liqun Xi & Ji-Ping Wang & Jonathan Widom, 2012. "A map of nucleosome positions in yeast at base-pair resolution," Nature, Nature, vol. 486(7404), pages 496-501, June.
    • Handle: RePEc:nat:nature:v:486:y:2012:i:7404:d:10.1038_nature11142
    DOI: 10.1038/nature11142
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    Cited by:

    1. Carlo Yague-Sanz & Valérie Migeot & Marc Larochelle & François Bachand & Maxime Wéry & Antonin Morillon & Damien Hermand, 2023. "Chromatin remodeling by Pol II primes efficient Pol III transcription," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    2. Behrouz Eslami-Mossallam & Raoul D Schram & Marco Tompitak & John van Noort & Helmut Schiessel, 2016. "Multiplexing Genetic and Nucleosome Positioning Codes: A Computational Approach," PLOS ONE, Public Library of Science, vol. 11(6), pages 1-14, June.

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