IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v477y2011i7365d10.1038_nature10402.html
   My bibliography  Save this article

Structures of the RNA-guided surveillance complex from a bacterial immune system

Author

Listed:
  • Blake Wiedenheft

    (Howard Hughes Medical Institute, University of California
    University of California)

  • Gabriel C. Lander

    (Lawrence Berkeley National Laboratory)

  • Kaihong Zhou

    (Howard Hughes Medical Institute, University of California
    University of California)

  • Matthijs M. Jore

    (Laboratory of Microbiology, Wageningen University, Dreijenplein 10)

  • Stan J. J. Brouns

    (Laboratory of Microbiology, Wageningen University, Dreijenplein 10)

  • John van der Oost

    (Laboratory of Microbiology, Wageningen University, Dreijenplein 10)

  • Jennifer A. Doudna

    (Howard Hughes Medical Institute, University of California
    University of California
    University of California
    Lawrence Berkeley National Laboratory)

  • Eva Nogales

    (Howard Hughes Medical Institute, University of California
    University of California
    Lawrence Berkeley National Laboratory)

Abstract

Key player in bacterial immunity Bacterial cells use CRISPRs (clustered regularly interspaced short palindromic repeats) to defend against invading phages. The central catalytic component in this process is Cascade, a 12-subunit complex consisting of proteins and RNA. The structure of Cascade, free and bound to target RNA, has now been solved by cryoelectron microscopy and three-dimensional reconstruction. These structures show the changes in architecture that are induced by target binding, and will assist future studies addressing how these conformational changes affect restriction of the phage.

Suggested Citation

  • Blake Wiedenheft & Gabriel C. Lander & Kaihong Zhou & Matthijs M. Jore & Stan J. J. Brouns & John van der Oost & Jennifer A. Doudna & Eva Nogales, 2011. "Structures of the RNA-guided surveillance complex from a bacterial immune system," Nature, Nature, vol. 477(7365), pages 486-489, September.
    • Handle: RePEc:nat:nature:v:477:y:2011:i:7365:d:10.1038_nature10402
    DOI: 10.1038/nature10402
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature10402
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature10402?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Kazuto Yoshimi & Kohei Takeshita & Noriyuki Kodera & Satomi Shibumura & Yuko Yamauchi & Mine Omatsu & Kenichi Umeda & Yayoi Kunihiro & Masaki Yamamoto & Tomoji Mashimo, 2022. "Dynamic mechanisms of CRISPR interference by Escherichia coli CRISPR-Cas3," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    2. Meiling Lu & Chenlin Yu & Yuwen Zhang & Wenjun Ju & Zhi Ye & Chenyang Hua & Jinze Mao & Chunyi Hu & Zhenhuang Yang & Yibei Xiao, 2024. "Structure and genome editing of type I-B CRISPR-Cas," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    3. Marius Rutkauskas & Inga Songailiene & Patrick Irmisch & Felix E. Kemmerich & Tomas Sinkunas & Virginijus Siksnys & Ralf Seidel, 2022. "A quantitative model for the dynamics of target recognition and off-target rejection by the CRISPR-Cas Cascade complex," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    4. Pierre Aldag & Marius Rutkauskas & Julene Madariaga-Marcos & Inga Songailiene & Tomas Sinkunas & Felix Kemmerich & Dominik Kauert & Virginijus Siksnys & Ralf Seidel, 2023. "Dynamic interplay between target search and recognition for a Type I CRISPR-Cas system," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:477:y:2011:i:7365:d:10.1038_nature10402. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.