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Structures of the RNA-guided surveillance complex from a bacterial immune system

Author

Listed:
  • Blake Wiedenheft

    (Howard Hughes Medical Institute, University of California
    University of California)

  • Gabriel C. Lander

    (Lawrence Berkeley National Laboratory)

  • Kaihong Zhou

    (Howard Hughes Medical Institute, University of California
    University of California)

  • Matthijs M. Jore

    (Laboratory of Microbiology, Wageningen University, Dreijenplein 10)

  • Stan J. J. Brouns

    (Laboratory of Microbiology, Wageningen University, Dreijenplein 10)

  • John van der Oost

    (Laboratory of Microbiology, Wageningen University, Dreijenplein 10)

  • Jennifer A. Doudna

    (Howard Hughes Medical Institute, University of California
    University of California
    University of California
    Lawrence Berkeley National Laboratory)

  • Eva Nogales

    (Howard Hughes Medical Institute, University of California
    University of California
    Lawrence Berkeley National Laboratory)

Abstract

Key player in bacterial immunity Bacterial cells use CRISPRs (clustered regularly interspaced short palindromic repeats) to defend against invading phages. The central catalytic component in this process is Cascade, a 12-subunit complex consisting of proteins and RNA. The structure of Cascade, free and bound to target RNA, has now been solved by cryoelectron microscopy and three-dimensional reconstruction. These structures show the changes in architecture that are induced by target binding, and will assist future studies addressing how these conformational changes affect restriction of the phage.

Suggested Citation

  • Blake Wiedenheft & Gabriel C. Lander & Kaihong Zhou & Matthijs M. Jore & Stan J. J. Brouns & John van der Oost & Jennifer A. Doudna & Eva Nogales, 2011. "Structures of the RNA-guided surveillance complex from a bacterial immune system," Nature, Nature, vol. 477(7365), pages 486-489, September.
    • Handle: RePEc:nat:nature:v:477:y:2011:i:7365:d:10.1038_nature10402
    DOI: 10.1038/nature10402
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    Cited by:

    1. Meiling Lu & Chenlin Yu & Yuwen Zhang & Wenjun Ju & Zhi Ye & Chenyang Hua & Jinze Mao & Chunyi Hu & Zhenhuang Yang & Yibei Xiao, 2024. "Structure and genome editing of type I-B CRISPR-Cas," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Marius Rutkauskas & Inga Songailiene & Patrick Irmisch & Felix E. Kemmerich & Tomas Sinkunas & Virginijus Siksnys & Ralf Seidel, 2022. "A quantitative model for the dynamics of target recognition and off-target rejection by the CRISPR-Cas Cascade complex," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    3. Pierre Aldag & Marius Rutkauskas & Julene Madariaga-Marcos & Inga Songailiene & Tomas Sinkunas & Felix Kemmerich & Dominik Kauert & Virginijus Siksnys & Ralf Seidel, 2023. "Dynamic interplay between target search and recognition for a Type I CRISPR-Cas system," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    4. Kazuto Yoshimi & Kohei Takeshita & Noriyuki Kodera & Satomi Shibumura & Yuko Yamauchi & Mine Omatsu & Kenichi Umeda & Yayoi Kunihiro & Masaki Yamamoto & Tomoji Mashimo, 2022. "Dynamic mechanisms of CRISPR interference by Escherichia coli CRISPR-Cas3," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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