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Induction of functional hepatocyte-like cells from mouse fibroblasts by defined factors

Author

Listed:
  • Pengyu Huang

    (Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy for Sciences, Yueyang Road 320, 200031 Shanghai, China)

  • Zhiying He

    (Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy for Sciences, Yueyang Road 320, 200031 Shanghai, China
    Second Military Medical University, 800 Xiangyin Road, 200433 Shanghai, China)

  • Shuyi Ji

    (Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy for Sciences, Yueyang Road 320, 200031 Shanghai, China)

  • Huawang Sun

    (Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy for Sciences, Yueyang Road 320, 200031 Shanghai, China)

  • Dao Xiang

    (Second Military Medical University, 800 Xiangyin Road, 200433 Shanghai, China)

  • Changcheng Liu

    (Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy for Sciences, Yueyang Road 320, 200031 Shanghai, China
    Second Military Medical University, 800 Xiangyin Road, 200433 Shanghai, China)

  • Yiping Hu

    (Second Military Medical University, 800 Xiangyin Road, 200433 Shanghai, China)

  • Xin Wang

    (Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy for Sciences, Yueyang Road 320, 200031 Shanghai, China
    University of Minnesota
    Stem Cell Institute, University of Minnesota)

  • Lijian Hui

    (Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy for Sciences, Yueyang Road 320, 200031 Shanghai, China)

Abstract

Direct routes to liver-like cells Two groups report new approaches that could lead to the generation of hepatocyte-like cells for liver engineering and regenerative medicine. Lijian Hui and colleagues use a combination of Gata4, Hnf1a and Foxa3 overexpression and p19Arf inactivation to convert mouse fibroblasts directly into induced hepatic (iHep) cells that have gene-expression profiles close to that of mature hepatocytes. Sayaka Sekiya and Atsushi Suzuki identify three combinations of two transcription factors, comprising Hnf4a plus Foxa1, Foxa2 or Foxa3, that can convert mouse embryonic and adult fibroblasts directly into functional iHep cells. Both groups show that when iHep cells are transplanted into mice with a gene deficiency that models liver injury, the cells are able to repopulate the livers and restore their function.

Suggested Citation

  • Pengyu Huang & Zhiying He & Shuyi Ji & Huawang Sun & Dao Xiang & Changcheng Liu & Yiping Hu & Xin Wang & Lijian Hui, 2011. "Induction of functional hepatocyte-like cells from mouse fibroblasts by defined factors," Nature, Nature, vol. 475(7356), pages 386-389, July.
    • Handle: RePEc:nat:nature:v:475:y:2011:i:7356:d:10.1038_nature10116
    DOI: 10.1038/nature10116
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    Cited by:

    1. Ren Guo & Mengmeng Jiang & Gang Wang & Bing Li & Xiaohui Jia & Yan Ai & Shanshan Chen & Peilan Tang & Aijie Liu & Qianting Yuan & Xin Xie, 2022. "IL6 supports long-term expansion of hepatocytes in vitro," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    2. Emre Bektik & Adrienne Dennis & Prateek Prasanna & Anant Madabhushi & Ji-Dong Fu, 2017. "Single cell qPCR reveals that additional HAND2 and microRNA-1 facilitate the early reprogramming progress of seven-factor-induced human myocytes," PLOS ONE, Public Library of Science, vol. 12(8), pages 1-16, August.
    3. Nana Yan & Hu Feng & Yongsen Sun & Ying Xin & Haihang Zhang & Hongjiang Lu & Jitan Zheng & Chenfei He & Zhenrui Zuo & Tanglong Yuan & Nana Li & Long Xie & Wu Wei & Yidi Sun & Erwei Zuo, 2023. "Cytosine base editors induce off-target mutations and adverse phenotypic effects in transgenic mice," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

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