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The mechanism of sodium and substrate release from the binding pocket of vSGLT

Author

Listed:
  • Akira Watanabe

    (University of California, Los Angeles)

  • Seungho Choe

    (University of Pittsburgh)

  • Vincent Chaptal

    (University of California, Los Angeles)

  • John M. Rosenberg

    (University of Pittsburgh
    University of Pittsburgh)

  • Ernest M. Wright

    (University of California, Los Angeles)

  • Michael Grabe

    (University of Pittsburgh
    University of Pittsburgh)

  • Jeff Abramson

    (University of California, Los Angeles)

Abstract

Sodium-coupled transport In membrane proteins with a 'LeuT-fold', it is not clear how ion- and substrate-transport are coupled. Watanabe et al. present a comprehensive study of the structure and biochemical properties of the sodium/galactose transporter (vSGLT) from Vibrio parahaemolyticus, and a new crystal structure of the 'inward-open' conformation. These experiments show that sodium exit causes a reorientation of transmembrane helix 1, opening an inner gate required for substrate exit, while also triggering minor rigid body movements in two sets of transmembrane helical bundles. This cascade of conformational changes is responsible for the proper timing of ion and substrate release.

Suggested Citation

  • Akira Watanabe & Seungho Choe & Vincent Chaptal & John M. Rosenberg & Ernest M. Wright & Michael Grabe & Jeff Abramson, 2010. "The mechanism of sodium and substrate release from the binding pocket of vSGLT," Nature, Nature, vol. 468(7326), pages 988-991, December.
    • Handle: RePEc:nat:nature:v:468:y:2010:i:7326:d:10.1038_nature09580
    DOI: 10.1038/nature09580
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    Cited by:

    1. Farha Khan & Matthias Elgeti & Samuel Grandfield & Aviv Paz & Fiona B. Naughton & Frank V. Marcoline & Thorsten Althoff & Natalia Ermolova & Ernest M. Wright & Wayne L. Hubbell & Michael Grabe & Jeff , 2023. "Membrane potential accelerates sugar uptake by stabilizing the outward facing conformation of the Na/glucose symporter vSGLT," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    2. Hyun Deok Song & Fangqiang Zhu, 2015. "Conformational Changes in Two Inter-Helical Loops of Mhp1 Membrane Transporter," PLOS ONE, Public Library of Science, vol. 10(7), pages 1-19, July.

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