IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v468y2010i7320d10.1038_nature09450.html
   My bibliography  Save this article

Video imaging of walking myosin V by high-speed atomic force microscopy

Author

Listed:
  • Noriyuki Kodera

    (Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan
    CREST, JST, Sanban-cho, Chiyoda-ku, Tokyo 102-0075, Japan)

  • Daisuke Yamamoto

    (Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan
    CREST, JST, Sanban-cho, Chiyoda-ku, Tokyo 102-0075, Japan)

  • Ryoki Ishikawa

    (Gunma University Graduate School of Medicine)

  • Toshio Ando

    (Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan
    CREST, JST, Sanban-cho, Chiyoda-ku, Tokyo 102-0075, Japan)

Abstract

The dynamic behaviour of myosin V molecules translocating along actin filaments has been mainly studied by optical microscopy. The processive hand-over-hand movement coupled with hydrolysis of adenosine triphosphate was thereby demonstrated. However, the protein molecules themselves are invisible in the observations and have therefore been visualized by electron microscopy in the stationary states. The concomitant assessment of structure and dynamics has been unfeasible, a situation prevailing throughout biological research. Here we directly visualize myosin V molecules walking along actin tracks, using high-speed atomic force microscopy. The high-resolution movies not only provide corroborative ‘visual evidence’ for previously speculated or demonstrated molecular behaviours, including lever-arm swing, but also reveal more detailed behaviours of the molecules, leading to a comprehensive understanding of the motor mechanism. Our direct and dynamic high-resolution visualization is a powerful new approach to studying the structure and dynamics of biomolecules in action.

Suggested Citation

  • Noriyuki Kodera & Daisuke Yamamoto & Ryoki Ishikawa & Toshio Ando, 2010. "Video imaging of walking myosin V by high-speed atomic force microscopy," Nature, Nature, vol. 468(7320), pages 72-76, November.
  • Handle: RePEc:nat:nature:v:468:y:2010:i:7320:d:10.1038_nature09450
    DOI: 10.1038/nature09450
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature09450
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.

    File URL: https://libkey.io/10.1038/nature09450?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Chou, Y.C., 2019. "Dynamical mechanism of stepping of the molecular motor myosin V along actin filament and simulation in an actual system," Physica A: Statistical Mechanics and its Applications, Elsevier, vol. 521(C), pages 399-405.
    2. Qing-Miao Nie & Akio Togashi & Takeshi N Sasaki & Mitsunori Takano & Masaki Sasai & Tomoki P Terada, 2014. "Coupling of Lever Arm Swing and Biased Brownian Motion in Actomyosin," PLOS Computational Biology, Public Library of Science, vol. 10(4), pages 1-13, April.
    3. Fang Jiao & François Dehez & Tao Ni & Xiulian Yu & Jeremy S. Dittman & Robert Gilbert & Christophe Chipot & Simon Scheuring, 2022. "Perforin-2 clockwise hand-over-hand pre-pore to pore transition mechanism," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    4. Rong Zhu & Daniel Canena & Mateusz Sikora & Miriam Klausberger & Hannah Seferovic & Ahmad Reza Mehdipour & Lisa Hain & Elisabeth Laurent & Vanessa Monteil & Gerald Wirnsberger & Ralph Wieneke & Robert, 2022. "Force-tuned avidity of spike variant-ACE2 interactions viewed on the single-molecule level," Nature Communications, Nature, vol. 13(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:468:y:2010:i:7320:d:10.1038_nature09450. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.