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Video imaging of walking myosin V by high-speed atomic force microscopy

Author

Listed:
  • Noriyuki Kodera

    (Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan
    CREST, JST, Sanban-cho, Chiyoda-ku, Tokyo 102-0075, Japan)

  • Daisuke Yamamoto

    (Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan
    CREST, JST, Sanban-cho, Chiyoda-ku, Tokyo 102-0075, Japan)

  • Ryoki Ishikawa

    (Gunma University Graduate School of Medicine)

  • Toshio Ando

    (Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan
    CREST, JST, Sanban-cho, Chiyoda-ku, Tokyo 102-0075, Japan)

Abstract

The dynamic behaviour of myosin V molecules translocating along actin filaments has been mainly studied by optical microscopy. The processive hand-over-hand movement coupled with hydrolysis of adenosine triphosphate was thereby demonstrated. However, the protein molecules themselves are invisible in the observations and have therefore been visualized by electron microscopy in the stationary states. The concomitant assessment of structure and dynamics has been unfeasible, a situation prevailing throughout biological research. Here we directly visualize myosin V molecules walking along actin tracks, using high-speed atomic force microscopy. The high-resolution movies not only provide corroborative ‘visual evidence’ for previously speculated or demonstrated molecular behaviours, including lever-arm swing, but also reveal more detailed behaviours of the molecules, leading to a comprehensive understanding of the motor mechanism. Our direct and dynamic high-resolution visualization is a powerful new approach to studying the structure and dynamics of biomolecules in action.

Suggested Citation

  • Noriyuki Kodera & Daisuke Yamamoto & Ryoki Ishikawa & Toshio Ando, 2010. "Video imaging of walking myosin V by high-speed atomic force microscopy," Nature, Nature, vol. 468(7320), pages 72-76, November.
    • Handle: RePEc:nat:nature:v:468:y:2010:i:7320:d:10.1038_nature09450
    DOI: 10.1038/nature09450
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    Cited by:

    1. Chou, Y.C., 2019. "Dynamical mechanism of stepping of the molecular motor myosin V along actin filament and simulation in an actual system," Physica A: Statistical Mechanics and its Applications, Elsevier, vol. 521(C), pages 399-405.
    2. Qing-Miao Nie & Akio Togashi & Takeshi N Sasaki & Mitsunori Takano & Masaki Sasai & Tomoki P Terada, 2014. "Coupling of Lever Arm Swing and Biased Brownian Motion in Actomyosin," PLOS Computational Biology, Public Library of Science, vol. 10(4), pages 1-13, April.
    3. Fang Jiao & François Dehez & Tao Ni & Xiulian Yu & Jeremy S. Dittman & Robert Gilbert & Christophe Chipot & Simon Scheuring, 2022. "Perforin-2 clockwise hand-over-hand pre-pore to pore transition mechanism," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    4. Rong Zhu & Daniel Canena & Mateusz Sikora & Miriam Klausberger & Hannah Seferovic & Ahmad Reza Mehdipour & Lisa Hain & Elisabeth Laurent & Vanessa Monteil & Gerald Wirnsberger & Ralph Wieneke & Robert, 2022. "Force-tuned avidity of spike variant-ACE2 interactions viewed on the single-molecule level," Nature Communications, Nature, vol. 13(1), pages 1-17, December.

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