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Genome remodelling in a basal-like breast cancer metastasis and xenograft

Author

Listed:
  • Li Ding

    (The Genome Center at Washington University,
    Department of Genetics,)

  • Matthew J. Ellis

    (Department of Medicine,
    Siteman Cancer Center,)

  • Shunqiang Li

    (Department of Medicine,)

  • David E. Larson

    (The Genome Center at Washington University,)

  • Ken Chen

    (The Genome Center at Washington University,)

  • John W. Wallis

    (The Genome Center at Washington University,
    Department of Genetics,)

  • Christopher C. Harris

    (The Genome Center at Washington University,)

  • Michael D. McLellan

    (The Genome Center at Washington University,)

  • Robert S. Fulton

    (The Genome Center at Washington University,
    Department of Genetics,)

  • Lucinda L. Fulton

    (The Genome Center at Washington University,
    Department of Genetics,)

  • Rachel M. Abbott

    (The Genome Center at Washington University,)

  • Jeremy Hoog

    (Department of Medicine,)

  • David J. Dooling

    (The Genome Center at Washington University,
    Department of Genetics,)

  • Daniel C. Koboldt

    (The Genome Center at Washington University,)

  • Heather Schmidt

    (The Genome Center at Washington University,)

  • Joelle Kalicki

    (The Genome Center at Washington University,)

  • Qunyuan Zhang

    (Department of Genetics,
    Division of Statistical Genomics,)

  • Lei Chen

    (The Genome Center at Washington University,)

  • Ling Lin

    (The Genome Center at Washington University,)

  • Michael C. Wendl

    (The Genome Center at Washington University,
    Department of Genetics,)

  • Joshua F. McMichael

    (The Genome Center at Washington University,)

  • Vincent J. Magrini

    (The Genome Center at Washington University,
    Department of Genetics,)

  • Lisa Cook

    (The Genome Center at Washington University,)

  • Sean D. McGrath

    (The Genome Center at Washington University,)

  • Tammi L. Vickery

    (The Genome Center at Washington University,)

  • Elizabeth Appelbaum

    (The Genome Center at Washington University,)

  • Katherine DeSchryver

    (Department of Medicine,)

  • Sherri Davies

    (Department of Medicine,)

  • Therese Guintoli

    (Department of Medicine,)

  • Li Lin

    (Department of Medicine,)

  • Robert Crowder

    (Department of Medicine,)

  • Yu Tao

    (Division of Biostatistics,)

  • Jacqueline E. Snider

    (Department of Medicine,)

  • Scott M. Smith

    (The Genome Center at Washington University,)

  • Adam F. Dukes

    (The Genome Center at Washington University,)

  • Gabriel E. Sanderson

    (The Genome Center at Washington University,)

  • Craig S. Pohl

    (The Genome Center at Washington University,)

  • Kim D. Delehaunty

    (The Genome Center at Washington University,)

  • Catrina C. Fronick

    (The Genome Center at Washington University,)

  • Kimberley A. Pape

    (The Genome Center at Washington University,)

  • Jerry S. Reed

    (The Genome Center at Washington University,)

  • Jody S. Robinson

    (The Genome Center at Washington University,)

  • Jennifer S. Hodges

    (The Genome Center at Washington University,)

  • William Schierding

    (The Genome Center at Washington University,)

  • Nathan D. Dees

    (The Genome Center at Washington University,)

  • Dong Shen

    (The Genome Center at Washington University,)

  • Devin P. Locke

    (The Genome Center at Washington University,)

  • Madeline E. Wiechert

    (The Genome Center at Washington University,)

  • James M. Eldred

    (The Genome Center at Washington University,)

  • Josh B. Peck

    (The Genome Center at Washington University,)

  • Benjamin J. Oberkfell

    (The Genome Center at Washington University,)

  • Justin T. Lolofie

    (The Genome Center at Washington University,)

  • Feiyu Du

    (The Genome Center at Washington University,)

  • Amy E. Hawkins

    (The Genome Center at Washington University,)

  • Michelle D. O’Laughlin

    (The Genome Center at Washington University,)

  • Kelly E. Bernard

    (The Genome Center at Washington University,)

  • Mark Cunningham

    (The Genome Center at Washington University,)

  • Glendoria Elliott

    (The Genome Center at Washington University,)

  • Mark D. Mason

    (The Genome Center at Washington University,)

  • Dominic M. Thompson Jr

    (Department of Surgery and the Young Women’s Breast Cancer Program,)

  • Jennifer L. Ivanovich

    (Department of Surgery and the Young Women’s Breast Cancer Program,)

  • Paul J. Goodfellow

    (Department of Surgery and the Young Women’s Breast Cancer Program,)

  • Charles M. Perou

    (Washington University School of Medicine, St Louis, Missouri 63108, USA)

  • George M. Weinstock

    (The Genome Center at Washington University,
    Department of Genetics,)

  • Rebecca Aft

    (Department of Surgery and the Young Women’s Breast Cancer Program,)

  • Mark Watson

    (Lineberger Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA)

  • Timothy J. Ley

    (The Genome Center at Washington University,
    Department of Genetics,
    Department of Medicine,
    Siteman Cancer Center,)

  • Richard K. Wilson

    (The Genome Center at Washington University,)

  • Elaine R. Mardis

    (The Genome Center at Washington University,
    Department of Genetics,
    Siteman Cancer Center,)

Abstract

Massively parallel DNA sequencing technologies provide an unprecedented ability to screen entire genomes for genetic changes associated with tumour progression. Here we describe the genomic analyses of four DNA samples from an African-American patient with basal-like breast cancer: peripheral blood, the primary tumour, a brain metastasis and a xenograft derived from the primary tumour. The metastasis contained two de novo mutations and a large deletion not present in the primary tumour, and was significantly enriched for 20 shared mutations. The xenograft retained all primary tumour mutations and displayed a mutation enrichment pattern that resembled the metastasis. Two overlapping large deletions, encompassing CTNNA1, were present in all three tumour samples. The differential mutation frequencies and structural variation patterns in metastasis and xenograft compared with the primary tumour indicate that secondary tumours may arise from a minority of cells within the primary tumour.

Suggested Citation

  • Li Ding & Matthew J. Ellis & Shunqiang Li & David E. Larson & Ken Chen & John W. Wallis & Christopher C. Harris & Michael D. McLellan & Robert S. Fulton & Lucinda L. Fulton & Rachel M. Abbott & Jeremy, 2010. "Genome remodelling in a basal-like breast cancer metastasis and xenograft," Nature, Nature, vol. 464(7291), pages 999-1005, April.
    • Handle: RePEc:nat:nature:v:464:y:2010:i:7291:d:10.1038_nature08989
    DOI: 10.1038/nature08989
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    Cited by:

    1. Naomi Kawashima & Yuichi Ishikawa & Jeong Hui Kim & Yoko Ushijima & Akimi Akashi & Yohei Yamaguchi & Hikaru Hattori & Marie Nakashima & Seara Ikeno & Rika Kihara & Takahiro Nishiyama & Takanobu Morish, 2022. "Comparison of clonal architecture between primary and immunodeficient mouse-engrafted acute myeloid leukemia cells," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    2. Qiuchen Guo & Milos Spasic & Adam G. Maynard & Gregory J. Goreczny & Amanuel Bizuayehu & Jessica F. Olive & Peter Galen & Sandra S. McAllister, 2022. "Clonal barcoding with qPCR detection enables live cell functional analyses for cancer research," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

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