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High-performance genetically targetable optical neural silencing by light-driven proton pumps

Author

Listed:
  • Brian Y. Chow

    (The MIT Media Laboratory, Synthetic Neurobiology Group
    Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA)

  • Xue Han

    (The MIT Media Laboratory, Synthetic Neurobiology Group
    Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA)

  • Allison S. Dobry

    (The MIT Media Laboratory, Synthetic Neurobiology Group
    Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA)

  • Xiaofeng Qian

    (The MIT Media Laboratory, Synthetic Neurobiology Group
    Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA)

  • Amy S. Chuong

    (The MIT Media Laboratory, Synthetic Neurobiology Group
    Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA)

  • Mingjie Li

    (The MIT Media Laboratory, Synthetic Neurobiology Group
    Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA)

  • Michael A. Henninger

    (The MIT Media Laboratory, Synthetic Neurobiology Group
    Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA)

  • Gabriel M. Belfort

    (Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA)

  • Yingxi Lin

    (Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA)

  • Patrick E. Monahan

    (The MIT Media Laboratory, Synthetic Neurobiology Group
    Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA)

  • Edward S. Boyden

    (The MIT Media Laboratory, Synthetic Neurobiology Group
    Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA)

Abstract

Light switch for neural circuits The experimental use of microbial opsins — light-sensitive ion channels — has ushered in a revolution in neuroscience, as they make it possible to modulate the activity of genetically targeted neurons in response to exogenous light. Now, Ed Boyden and colleagues have screened archaebacteria, bacteria, plants and fungi for opsins with novel properties and have found a fundamentally new mechanism for neural control: light-driven proton pumping. Although protons are not used natively as charge carriers by neural systems, light-driven proton pumping by archaerhodopsin-3 from Halorubrum sodomense mediates powerful neural silencing in response to light. And a proton pump from the fungus Leptosphaeria maculans enables neural silencing by blue light. The use of these reagents will facilitate the shutdown of neural circuits with light as a tool for studying the role of neural circuits in behaviour and pathology.

Suggested Citation

  • Brian Y. Chow & Xue Han & Allison S. Dobry & Xiaofeng Qian & Amy S. Chuong & Mingjie Li & Michael A. Henninger & Gabriel M. Belfort & Yingxi Lin & Patrick E. Monahan & Edward S. Boyden, 2010. "High-performance genetically targetable optical neural silencing by light-driven proton pumps," Nature, Nature, vol. 463(7277), pages 98-102, January.
    • Handle: RePEc:nat:nature:v:463:y:2010:i:7277:d:10.1038_nature08652
    DOI: 10.1038/nature08652
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    Cited by:

    1. Aki Takahashi & Romain Durand-de Cuttoli & Meghan E. Flanigan & Emi Hasegawa & Tomomi Tsunematsu & Hossein Aleyasin & Yoan Cherasse & Ken Miya & Takuya Okada & Kazuko Keino-Masu & Koshiro Mitsui & Lon, 2022. "Lateral habenula glutamatergic neurons projecting to the dorsal raphe nucleus promote aggressive arousal in mice," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    2. Lizhu Li & Lihui Lu & Yuqi Ren & Guo Tang & Yu Zhao & Xue Cai & Zhao Shi & He Ding & Changbo Liu & Dali Cheng & Yang Xie & Huachun Wang & Xin Fu & Lan Yin & Minmin Luo & Xing Sheng, 2022. "Colocalized, bidirectional optogenetic modulations in freely behaving mice with a wireless dual-color optoelectronic probe," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    3. Stanislav Ott & Sangyu Xu & Nicole Lee & Ivan Hong & Jonathan Anns & Danesha Devini Suresh & Zhiyi Zhang & Xianyuan Zhang & Raihanah Harion & Weiying Ye & Vaishnavi Chandramouli & Suresh Jesuthasan & , 2024. "Kalium channelrhodopsins effectively inhibit neurons," Nature Communications, Nature, vol. 15(1), pages 1-21, December.
    4. Leonardo Barneschi & Emanuele Marsili & Laura Pedraza-González & Daniele Padula & Luca De Vico & Danil Kaliakin & Alejandro Blanco-González & Nicolas Ferré & Miquel Huix-Rotllant & Michael Filatov & M, 2022. "On the fluorescence enhancement of arch neuronal optogenetic reporters," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    5. Ya-Nan Zhao & Jian-Bo Jiang & Shi-Yuan Tao & Yang Zhang & Ze-Ka Chen & Wei-Min Qu & Zhi-Li Huang & Su-Rong Yang, 2022. "GABAergic neurons in the rostromedial tegmental nucleus are essential for rapid eye movement sleep suppression," Nature Communications, Nature, vol. 13(1), pages 1-18, December.

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