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On the fluorescence enhancement of arch neuronal optogenetic reporters

Author

Listed:
  • Leonardo Barneschi

    (Università di Siena)

  • Emanuele Marsili

    (Università di Siena
    University of Durham, Department of Chemistry
    University of Bristol)

  • Laura Pedraza-González

    (Università di Siena
    Dipartimento di Chimica e Chimica Industriale, Università di Pisa)

  • Daniele Padula

    (Università di Siena)

  • Luca De Vico

    (Università di Siena)

  • Danil Kaliakin

    (Bowling Green State University)

  • Alejandro Blanco-González

    (Bowling Green State University)

  • Nicolas Ferré

    (Institut de Chimie Radicalaire (UMR-7273), Aix-Marseille Université, CNRS)

  • Miquel Huix-Rotllant

    (Institut de Chimie Radicalaire (UMR-7273), Aix-Marseille Université, CNRS)

  • Michael Filatov

    (Kyungpook National University)

  • Massimo Olivucci

    (Università di Siena
    Bowling Green State University
    University of Strasbourg Institute for Advanced Studies)

Abstract

The lack of a theory capable of connecting the amino acid sequence of a light-absorbing protein with its fluorescence brightness is hampering the development of tools for understanding neuronal communications. Here we demonstrate that a theory can be established by constructing quantum chemical models of a set of Archaerhodopsin reporters in their electronically excited state. We found that the experimentally observed increase in fluorescence quantum yield is proportional to the computed decrease in energy difference between the fluorescent state and a nearby photoisomerization channel leading to an exotic diradical of the protein chromophore. This finding will ultimately support the development of technologies for searching novel fluorescent rhodopsin variants and unveil electrostatic changes that make light emission brighter and brighter.

Suggested Citation

  • Leonardo Barneschi & Emanuele Marsili & Laura Pedraza-González & Daniele Padula & Luca De Vico & Danil Kaliakin & Alejandro Blanco-González & Nicolas Ferré & Miquel Huix-Rotllant & Michael Filatov & M, 2022. "On the fluorescence enhancement of arch neuronal optogenetic reporters," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33993-4
    DOI: 10.1038/s41467-022-33993-4
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    References listed on IDEAS

    as
    1. Brian Y. Chow & Xue Han & Allison S. Dobry & Xiaofeng Qian & Amy S. Chuong & Mingjie Li & Michael A. Henninger & Gabriel M. Belfort & Yingxi Lin & Patrick E. Monahan & Edward S. Boyden, 2010. "High-performance genetically targetable optical neural silencing by light-driven proton pumps," Nature, Nature, vol. 463(7277), pages 98-102, January.
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