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Structural basis of abscisic acid signalling

Author

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  • Ken-ichi Miyazono

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Takuya Miyakawa

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Yoriko Sawano

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Keiko Kubota

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Hee-Jin Kang

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Atsuko Asano

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Yumiko Miyauchi

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Mihoko Takahashi

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Yuehua Zhi

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Yasunari Fujita

    (Japan International Research Center for Agricultural Sciences)

  • Takuya Yoshida

    (Japan International Research Center for Agricultural Sciences
    Laboratory of Plant Molecular Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Ken-Suke Kodaira

    (Japan International Research Center for Agricultural Sciences
    Laboratory of Plant Molecular Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Kazuko Yamaguchi-Shinozaki

    (Japan International Research Center for Agricultural Sciences
    Laboratory of Plant Molecular Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo)

  • Masaru Tanokura

    (Graduate School of Agricultural and Life Sciences, The University of Tokyo)

Abstract

The phytohormone abscisic acid (ABA) mediates the adaptation of plants to environmental stresses such as drought and regulates developmental signals such as seed maturation. Within plants, the PYR/PYL/RCAR family of START proteins receives ABA to inhibit the phosphatase activity of the group-A protein phosphatases 2C (PP2Cs), which are major negative regulators in ABA signalling. Here we present the crystal structures of the ABA receptor PYL1 bound with (+)-ABA, and the complex formed by the further binding of (+)-ABA-bound PYL1 with the PP2C protein ABI1. PYL1 binds (+)-ABA using the START-protein-specific ligand-binding site, thereby forming a hydrophobic pocket on the surface of the closed lid. (+)-ABA-bound PYL1 tightly interacts with a PP2C domain of ABI1 by using the hydrophobic pocket to cover the active site of ABI1 like a plug. Our results reveal the structural basis of the mechanism of (+)-ABA-dependent inhibition of ABI1 by PYL1 in ABA signalling.

Suggested Citation

  • Ken-ichi Miyazono & Takuya Miyakawa & Yoriko Sawano & Keiko Kubota & Hee-Jin Kang & Atsuko Asano & Yumiko Miyauchi & Mihoko Takahashi & Yuehua Zhi & Yasunari Fujita & Takuya Yoshida & Ken-Suke Kodaira, 2009. "Structural basis of abscisic acid signalling," Nature, Nature, vol. 462(7273), pages 609-614, December.
    • Handle: RePEc:nat:nature:v:462:y:2009:i:7273:d:10.1038_nature08583
    DOI: 10.1038/nature08583
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    Citations

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    Cited by:

    1. Zhang You & Shiyuan Guo & Qiao Li & Yanjun Fang & Panpan Huang & Chuanfeng Ju & Cun Wang, 2023. "The CBL1/9-CIPK1 calcium sensor negatively regulates drought stress by phosphorylating the PYLs ABA receptor," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Junyao Lu & Ning Li & Gaojian Li & Ziang Tian & Lianping Shi & Yan Wang & Yingao Cai & Kaiyuan Zhang & Wanting Sun & Danyang Wang & Jinxin Lin & Jinguang Huang & Changai Wu & Kang Yan & Shizhong Zhang, 2024. "N-glycosylation of SnRK2s affects NADPH maintenance in peroxisomes during prolonged ABA signalling," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    3. Xi Wang & Fan Zheng & Yuan-yuan Yi & Gao-yuan Wang & Li-xin Hong & Dannel McCollum & Chuanhai Fu & Yamei Wang & Quan-wen Jin, 2022. "Ubiquitination of CLIP-170 family protein restrains polarized growth upon DNA replication stress," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    4. Yage Ding & Cristina Tous & Jaehoon Choi & Jingyao Chen & Wilson W. Wong, 2024. "Orthogonal inducible control of Cas13 circuits enables programmable RNA regulation in mammalian cells," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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