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Membrane scission by the ESCRT-III complex

Author

Listed:
  • Thomas Wollert

    (Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, and,)

  • Christian Wunder

    (Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA)

  • Jennifer Lippincott-Schwartz

    (Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA)

  • James H. Hurley

    (Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, and,)

Abstract

The endosomal sorting complex required for transport (ESCRT) system is essential for multivesicular body biogenesis, in which cargo sorting is coupled to the invagination and scission of intralumenal vesicles. The ESCRTs are also needed for budding of enveloped viruses including human immunodeficiency virus 1, and for membrane abscission in cytokinesis. In Saccharomyces cerevisiae, ESCRT-III consists of Vps20, Snf7, Vps24 and Vps2 (also known as Did4), which assemble in that order and require the ATPase Vps4 for their disassembly. In this study, the ESCRT-III-dependent budding and scission of intralumenal vesicles into giant unilamellar vesicles was reconstituted and visualized by fluorescence microscopy. Here we show that three subunits of ESCRT-III, Vps20, Snf7 and Vps24, are sufficient to detach intralumenal vesicles. Vps2, the ESCRT-III subunit responsible for recruiting Vps4, and the ATPase activity of Vps4 were required for ESCRT-III recycling and supported additional rounds of budding. The minimum set of ESCRT-III and Vps4 proteins capable of multiple cycles of vesicle detachment corresponds to the ancient set of ESCRT proteins conserved from archaea to animals.

Suggested Citation

  • Thomas Wollert & Christian Wunder & Jennifer Lippincott-Schwartz & James H. Hurley, 2009. "Membrane scission by the ESCRT-III complex," Nature, Nature, vol. 458(7235), pages 172-177, March.
    • Handle: RePEc:nat:nature:v:458:y:2009:i:7235:d:10.1038_nature07836
    DOI: 10.1038/nature07836
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    Cited by:

    1. Nebojsa Jukic & Alma P. Perrino & Frédéric Humbert & Aurélien Roux & Simon Scheuring, 2022. "Snf7 spirals sense and alter membrane curvature," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    2. Gyu Ik Jung & Daniela Londoño-Vásquez & Sungjin Park & Ahna R. Skop & Ahmed Z. Balboula & Karen Schindler, 2023. "An oocyte meiotic midbody cap is required for developmental competence in mice," Nature Communications, Nature, vol. 14(1), pages 1-13, December.

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