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The complete genome of an individual by massively parallel DNA sequencing

Author

Listed:
  • David A. Wheeler

    (Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA)

  • Maithreyan Srinivasan

    (454 Life Sciences, Roche Diagnostics, 20 Commercial Street, Bradford, Connecticut 06405, USA)

  • Michael Egholm

    (454 Life Sciences, Roche Diagnostics, 20 Commercial Street, Bradford, Connecticut 06405, USA)

  • Yufeng Shen

    (Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA)

  • Lei Chen

    (Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA)

  • Amy McGuire

    (Center for Ethics and Health Policy, Baylor College of Medicine, One Baylor Plaza, Houston Texas 77030, USA)

  • Wen He

    (454 Life Sciences, Roche Diagnostics, 20 Commercial Street, Bradford, Connecticut 06405, USA)

  • Yi-Ju Chen

    (454 Life Sciences, Roche Diagnostics, 20 Commercial Street, Bradford, Connecticut 06405, USA)

  • Vinod Makhijani

    (454 Life Sciences, Roche Diagnostics, 20 Commercial Street, Bradford, Connecticut 06405, USA)

  • G. Thomas Roth

    (454 Life Sciences, Roche Diagnostics, 20 Commercial Street, Bradford, Connecticut 06405, USA)

  • Xavier Gomes

    (454 Life Sciences, Roche Diagnostics, 20 Commercial Street, Bradford, Connecticut 06405, USA)

  • Karrie Tartaro

    (454 Life Sciences, Roche Diagnostics, 20 Commercial Street, Bradford, Connecticut 06405, USA
    Present addresses: Molecular Imaging Systems, Carestream Health, Inc., 4 Science Park, New Haven, Connecticut 06511, USA (K.T.); Rothberg Institute for Childhood Diseases, 530 Whitfield Street, Guilford, Connecticut 06437, USA (J.M.R.).)

  • Faheem Niazi

    (454 Life Sciences, Roche Diagnostics, 20 Commercial Street, Bradford, Connecticut 06405, USA)

  • Cynthia L. Turcotte

    (454 Life Sciences, Roche Diagnostics, 20 Commercial Street, Bradford, Connecticut 06405, USA)

  • Gerard P. Irzyk

    (454 Life Sciences, Roche Diagnostics, 20 Commercial Street, Bradford, Connecticut 06405, USA)

  • James R. Lupski

    (Baylor College of Medicine, One Baylor Plaza, Houston Texas 77030, USA
    Baylor College of Medicine, One Baylor Plaza, Houston Texas 77030, USA
    Texas Children’s Hospital, Texas Medical Center, Houston, Texas 77030, USA)

  • Craig Chinault

    (Baylor College of Medicine, One Baylor Plaza, Houston Texas 77030, USA)

  • Xing-zhi Song

    (Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA)

  • Yue Liu

    (Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA)

  • Ye Yuan

    (Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA)

  • Lynne Nazareth

    (Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA)

  • Xiang Qin

    (Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA)

  • Donna M. Muzny

    (Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA)

  • Marcel Margulies

    (454 Life Sciences, Roche Diagnostics, 20 Commercial Street, Bradford, Connecticut 06405, USA)

  • George M. Weinstock

    (Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Baylor College of Medicine, One Baylor Plaza, Houston Texas 77030, USA)

  • Richard A. Gibbs

    (Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA
    Baylor College of Medicine, One Baylor Plaza, Houston Texas 77030, USA)

  • Jonathan M. Rothberg

    (454 Life Sciences, Roche Diagnostics, 20 Commercial Street, Bradford, Connecticut 06405, USA
    Present addresses: Molecular Imaging Systems, Carestream Health, Inc., 4 Science Park, New Haven, Connecticut 06511, USA (K.T.); Rothberg Institute for Childhood Diseases, 530 Whitfield Street, Guilford, Connecticut 06437, USA (J.M.R.).)

Abstract

One man's genome Next-generation sequencing technologies are revolutionizing human genomics, promising to yield draft genomes cheaply and quickly. One such technology has now been used to analyse much of the genetic code of a single individual — who happens to be James D. Watson. The procedure, which involves no cloning of the genomic DNA, makes use of the latest 454 parallel sequencing instrument. The sequence cost less than US$1 million (and a mere two months) to produce, compared to the approximately US$100 million reported for sequencing Craig Venter's genome by traditional methods. Still a major undertaking, but another step towards the goal of 'personalized genomes' and 'personalized medicine'.

Suggested Citation

  • David A. Wheeler & Maithreyan Srinivasan & Michael Egholm & Yufeng Shen & Lei Chen & Amy McGuire & Wen He & Yi-Ju Chen & Vinod Makhijani & G. Thomas Roth & Xavier Gomes & Karrie Tartaro & Faheem Niazi, 2008. "The complete genome of an individual by massively parallel DNA sequencing," Nature, Nature, vol. 452(7189), pages 872-876, April.
    • Handle: RePEc:nat:nature:v:452:y:2008:i:7189:d:10.1038_nature06884
    DOI: 10.1038/nature06884
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    Cited by:

    1. Richard Jiang & Prashant Singh & Fredrik Wrede & Andreas Hellander & Linda Petzold, 2022. "Identification of dynamic mass-action biochemical reaction networks using sparse Bayesian methods," PLOS Computational Biology, Public Library of Science, vol. 18(1), pages 1-21, January.
    2. Jiang Du & Robert D Bjornson & Zhengdong D Zhang & Yong Kong & Michael Snyder & Mark B Gerstein, 2009. "Integrating Sequencing Technologies in Personal Genomics: Optimal Low Cost Reconstruction of Structural Variants," PLOS Computational Biology, Public Library of Science, vol. 5(7), pages 1-15, July.
    3. Kirk E Lohmueller & Anders Albrechtsen & Yingrui Li & Su Yeon Kim & Thorfinn Korneliussen & Nicolas Vinckenbosch & Geng Tian & Emilia Huerta-Sanchez & Alison F Feder & Niels Grarup & Torben Jørgensen , 2011. "Natural Selection Affects Multiple Aspects of Genetic Variation at Putatively Neutral Sites across the Human Genome," PLOS Genetics, Public Library of Science, vol. 7(10), pages 1-15, October.

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