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Shugoshin collaborates with protein phosphatase 2A to protect cohesin

Author

Listed:
  • Tomoya S. Kitajima

    (Institute of Molecular and Cellular Biosciences)

  • Takeshi Sakuno

    (Institute of Molecular and Cellular Biosciences
    SORST, Japan Science and Technology Agency)

  • Kei-ichiro Ishiguro

    (Institute of Molecular and Cellular Biosciences)

  • Shun-ichiro Iemura

    (Biological Information Research Center)

  • Tohru Natsume

    (Biological Information Research Center)

  • Shigehiro A. Kawashima

    (Institute of Molecular and Cellular Biosciences
    University of Tokyo)

  • Yoshinori Watanabe

    (Institute of Molecular and Cellular Biosciences
    University of Tokyo
    SORST, Japan Science and Technology Agency)

Abstract

Sister chromatid cohesion, mediated by a complex called cohesin, is crucial—particularly at centromeres—for proper chromosome segregation in mitosis and meiosis. In animal mitotic cells, phosphorylation of cohesin promotes its dissociation from chromosomes, but centromeric cohesin is protected by shugoshin until kinetochores are properly captured by the spindle microtubules. However, the mechanism of shugoshin-dependent protection of cohesin is unknown. Here we find a specific subtype of serine/threonine protein phosphatase 2A (PP2A) associating with human shugoshin. PP2A colocalizes with shugoshin at centromeres and is required for centromeric protection. Purified shugoshin complex has an ability to reverse the phosphorylation of cohesin in vitro, suggesting that dephosphorylation of cohesin is the mechanism of protection at centromeres. Meiotic shugoshin of fission yeast also associates with PP2A, with both proteins collaboratively protecting Rec8-containing cohesin at centromeres. Thus, we have revealed a conserved mechanism of centromeric protection of eukaryotic chromosomes in mitosis and meiosis.

Suggested Citation

  • Tomoya S. Kitajima & Takeshi Sakuno & Kei-ichiro Ishiguro & Shun-ichiro Iemura & Tohru Natsume & Shigehiro A. Kawashima & Yoshinori Watanabe, 2006. "Shugoshin collaborates with protein phosphatase 2A to protect cohesin," Nature, Nature, vol. 441(7089), pages 46-52, May.
    • Handle: RePEc:nat:nature:v:441:y:2006:i:7089:d:10.1038_nature04663
    DOI: 10.1038/nature04663
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    Cited by:

    1. Ignacio Prusén Mota & Marta Galova & Alexander Schleiffer & Tan-Trung Nguyen & Ines Kovacikova & Carolina Farias Saad & Gabriele Litos & Tomoko Nishiyama & Juraj Gregan & Jan-Michael Peters & Peter Sc, 2024. "Sororin is an evolutionary conserved antagonist of WAPL," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    2. A. Danylevska & J. Sebestova, 2013. "Causes and consequences of maternal age-related aneuploidy in oocytes: a review," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 58(2), pages 65-72.

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