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STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis

Author

Listed:
  • Katrin I. Willig

    (Departments of NanoBiophotonics)

  • Silvio O. Rizzoli

    (Max Planck Institute for Biophysical Chemistry)

  • Volker Westphal

    (Departments of NanoBiophotonics)

  • Reinhard Jahn

    (Max Planck Institute for Biophysical Chemistry)

  • Stefan W. Hell

    (Departments of NanoBiophotonics)

Abstract

Single-synapse microscopy STED (stimulated emission depletion) microscopy is an emergent light microscopy technique that overcomes the diffraction barrier limiting the resolution of conventional fluorescence microscopes. This brings structures the size of the synaptic vesicles (40 nm) into the picture. Now STED microscopy has been used to resolve individual synaptic vesicles in single synapses of the mammalian central nervous system for the first time. The synaptic vesicle recycling that is central to neurotransmitter release has been intensely studied for more than 30 years, but a major question has remained unanswered: do their components diffuse on the plasma membrane, or do they remain together? STED microscopy reveals that at least one main component, synaptotagmin-1, remains clustered after exocytosis and is recycled en bloc.

Suggested Citation

  • Katrin I. Willig & Silvio O. Rizzoli & Volker Westphal & Reinhard Jahn & Stefan W. Hell, 2006. "STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis," Nature, Nature, vol. 440(7086), pages 935-939, April.
    • Handle: RePEc:nat:nature:v:440:y:2006:i:7086:d:10.1038_nature04592
    DOI: 10.1038/nature04592
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    Cited by:

    1. Jan-Erik Bredfeldt & Joanna Oracz & Kamila A. Kiszka & Thea Moosmayer & Michael Weber & Steffen J. Sahl & Stefan W. Hell, 2024. "Bleaching protection and axial sectioning in fluorescence nanoscopy through two-photon activation at 515 nm," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    2. Pengcheng Huo & Wei Chen & Zixuan Zhang & Yanzeng Zhang & Mingze Liu & Peicheng Lin & Hui Zhang & Zhaoxian Chen & Henri Lezec & Wenqi Zhu & Amit Agrawal & Chao Peng & Yanqing Lu & Ting Xu, 2024. "Observation of spatiotemporal optical vortices enabled by symmetry-breaking slanted nanograting," Nature Communications, Nature, vol. 15(1), pages 1-8, December.
    3. Ling-Gang Wu & Chung Yu Chan, 2024. "Membrane transformations of fusion and budding," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    4. Atsushi Yamagata & Kaori Ito & Takehiro Suzuki & Naoshi Dohmae & Tohru Terada & Mikako Shirouzu, 2024. "Structural basis for antiepileptic drugs and botulinum neurotoxin recognition of SV2A," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    5. Rui Pu & Qiuqiang Zhan & Xingyun Peng & Siying Liu & Xin Guo & Liangliang Liang & Xian Qin & Ziqing Winston Zhao & Xiaogang Liu, 2022. "Super-resolution microscopy enabled by high-efficiency surface-migration emission depletion," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    6. Xin Guo & Rui Pu & Zhimin Zhu & Shuqian Qiao & Yusen Liang & Bingru Huang & Haichun Liu & Lucía Labrador-Páez & Uliana Kostiv & Pu Zhao & Qiusheng Wu & Jerker Widengren & Qiuqiang Zhan, 2022. "Achieving low-power single-wavelength-pair nanoscopy with NIR-II continuous-wave laser for multi-chromatic probes," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    7. Christiaan N. Hulleman & Rasmus Ø. Thorsen & Eugene Kim & Cees Dekker & Sjoerd Stallinga & Bernd Rieger, 2021. "Simultaneous orientation and 3D localization microscopy with a Vortex point spread function," Nature Communications, Nature, vol. 12(1), pages 1-14, December.

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