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Achieving low-power single-wavelength-pair nanoscopy with NIR-II continuous-wave laser for multi-chromatic probes

Author

Listed:
  • Xin Guo

    (South China Normal University)

  • Rui Pu

    (South China Normal University)

  • Zhimin Zhu

    (South China Normal University)

  • Shuqian Qiao

    (South China Normal University)

  • Yusen Liang

    (South China Normal University)

  • Bingru Huang

    (South China Normal University)

  • Haichun Liu

    (KTH Royal Institute of Technology)

  • Lucía Labrador-Páez

    (KTH Royal Institute of Technology)

  • Uliana Kostiv

    (KTH Royal Institute of Technology)

  • Pu Zhao

    (South China Normal University)

  • Qiusheng Wu

    (South China Normal University)

  • Jerker Widengren

    (KTH Royal Institute of Technology)

  • Qiuqiang Zhan

    (South China Normal University
    South China Normal University)

Abstract

Stimulated emission depletion (STED) microscopy is a powerful diffraction-unlimited technique for fluorescence imaging. Despite its rapid evolution, STED fundamentally suffers from high-intensity light illumination, sophisticated probe-defined laser schemes, and limited photon budget of the probes. Here, we demonstrate a versatile strategy, stimulated-emission induced excitation depletion (STExD), to deplete the emission of multi-chromatic probes using a single pair of low-power, near-infrared (NIR), continuous-wave (CW) lasers with fixed wavelengths. With the effect of cascade amplified depletion in lanthanide upconversion systems, we achieve emission inhibition for a wide range of emitters (e.g., Nd3+, Yb3+, Er3+, Ho3+, Pr3+, Eu3+, Tm3+, Gd3+, and Tb3+) by manipulating their common sensitizer, i.e., Nd3+ ions, using a 1064-nm laser. With NaYF4:Nd nanoparticles, we demonstrate an ultrahigh depletion efficiency of 99.3 ± 0.3% for the 450 nm emission with a low saturation intensity of 23.8 ± 0.4 kW cm−2. We further demonstrate nanoscopic imaging with a series of multi-chromatic nanoprobes with a lateral resolution down to 34 nm, two-color STExD imaging, and subcellular imaging of the immunolabelled actin filaments. The strategy expounded here promotes single wavelength-pair nanoscopy for multi-chromatic probes and for multi-color imaging under low-intensity-level NIR-II CW laser depletion.

Suggested Citation

  • Xin Guo & Rui Pu & Zhimin Zhu & Shuqian Qiao & Yusen Liang & Bingru Huang & Haichun Liu & Lucía Labrador-Páez & Uliana Kostiv & Pu Zhao & Qiusheng Wu & Jerker Widengren & Qiuqiang Zhan, 2022. "Achieving low-power single-wavelength-pair nanoscopy with NIR-II continuous-wave laser for multi-chromatic probes," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30114-z
    DOI: 10.1038/s41467-022-30114-z
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    References listed on IDEAS

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    1. Katrin I. Willig & Silvio O. Rizzoli & Volker Westphal & Reinhard Jahn & Stefan W. Hell, 2006. "STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis," Nature, Nature, vol. 440(7086), pages 935-939, April.
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