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Nanospring behaviour of ankyrin repeats

Author

Listed:
  • Gwangrog Lee

    (Duke University)

  • Khadar Abdi

    (Duke University Medical Center)

  • Yong Jiang

    (Duke University)

  • Peter Michaely

    (University of Texas Southwestern Medical Center)

  • Vann Bennett

    (Duke University Medical Center)

  • Piotr E. Marszalek

    (Duke University)

Abstract

Ankyrin repeats are an amino-acid motif believed to function in protein recognition; they are present in tandem copies in diverse proteins in nearly all phyla1. Ankyrin repeats contain antiparallel α-helices that can stack to form a superhelical spiral2. Visual inspection of the extrapolated structure of 24 ankyrin-R repeats2 indicates the possibility of spring-like behaviour of the putative superhelix. Moreover, stacks of 17–29 ankyrin repeats in the cytoplasmic domains of transient receptor potential (TRP) channels have been identified as candidates for a spring that gates mechanoreceptors in hair cells as well as in Drosophila bristles3,4,5. Here we report that tandem ankyrin repeats exhibit tertiary-structure-based elasticity and behave as a linear and fully reversible spring in single-molecule measurements by atomic force microscopy. We also observe an unexpected ability of unfolded repeats to generate force during refolding, and report the first direct measurement of the refolding force of a protein domain. Thus, we show that one of the most common amino-acid motifs has spring properties that could be important in mechanotransduction and in the design of nanodevices.

Suggested Citation

  • Gwangrog Lee & Khadar Abdi & Yong Jiang & Peter Michaely & Vann Bennett & Piotr E. Marszalek, 2006. "Nanospring behaviour of ankyrin repeats," Nature, Nature, vol. 440(7081), pages 246-249, March.
    • Handle: RePEc:nat:nature:v:440:y:2006:i:7081:d:10.1038_nature04437
    DOI: 10.1038/nature04437
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    Cited by:

    1. A. Mills & N. Aissaoui & D. Maurel & J. Elezgaray & F. Morvan & J. J. Vasseur & E. Margeat & R. B. Quast & J. Lai Kee-Him & N. Saint & C. Benistant & A. Nord & F. Pedaci & G. Bellot, 2022. "A modular spring-loaded actuator for mechanical activation of membrane proteins," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    2. Nisha Arora & Jagadish P. Hazra & Sandip Roy & Gaurav K. Bhati & Sarika Gupta & K. P. Yogendran & Abhishek Chaudhuri & Amin Sagar & Sabyasachi Rakshit, 2024. "Emergence of slip-ideal-slip behavior in tip-links serve as force filters of sound in hearing," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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