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Functional genomics reveals genes involved in protein secretion and Golgi organization

Author

Listed:
  • Frederic Bard

    (University of California San Diego)

  • Laetitia Casano

    (University of California San Diego)

  • Arrate Mallabiabarrena

    (University of California San Diego)

  • Erin Wallace

    (University of California San Diego)

  • Kota Saito

    (University of California San Diego)

  • Hitoshi Kitayama

    (University of California San Diego)

  • Gianni Guizzunti

    (University of California San Diego)

  • Yue Hu

    (University of California San Diego)

  • Franz Wendler

    (the Ridgeway Mill Hill)

  • Ramanuj DasGupta

    (Harvard Medical School)

  • Norbert Perrimon

    (Harvard Medical School)

  • Vivek Malhotra

    (University of California San Diego)

Abstract

Yeast genetics and in vitro biochemical analysis have identified numerous genes involved in protein secretion1,2. As compared with yeast, however, the metazoan secretory pathway is more complex and many mechanisms that regulate organization of the Golgi apparatus remain poorly characterized. We performed a genome-wide RNA-mediated interference screen in a Drosophila cell line to identify genes required for constitutive protein secretion. We then classified the genes on the basis of the effect of their depletion on organization of the Golgi membranes. Here we show that depletion of class A genes redistributes Golgi membranes into the endoplasmic reticulum, depletion of class B genes leads to Golgi fragmentation, depletion of class C genes leads to aggregation of Golgi membranes, and depletion of class D genes causes no obvious change. Of the 20 new gene products characterized so far, several localize to the Golgi membranes and the endoplasmic reticulum.

Suggested Citation

  • Frederic Bard & Laetitia Casano & Arrate Mallabiabarrena & Erin Wallace & Kota Saito & Hitoshi Kitayama & Gianni Guizzunti & Yue Hu & Franz Wendler & Ramanuj DasGupta & Norbert Perrimon & Vivek Malhot, 2006. "Functional genomics reveals genes involved in protein secretion and Golgi organization," Nature, Nature, vol. 439(7076), pages 604-607, February.
    • Handle: RePEc:nat:nature:v:439:y:2006:i:7076:d:10.1038_nature04377
    DOI: 10.1038/nature04377
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    Cited by:

    1. Zhi Feng & Shengnan Liu & Ming Su & Chunyu Song & Chenyu Lin & Fangying Zhao & Yang Li & Xianyan Zeng & Yong Zhu & Yu Hou & Chunguang Ren & Huan Zhang & Ping Yi & Yong Ji & Chao Wang & Hongtao Li & Mi, 2024. "TANGO6 regulates cell proliferation via COPI vesicle-mediated RPB2 nuclear entry," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    2. Ishier Raote & Ann-Helen Rosendahl & Hanna-Maria Häkkinen & Carina Vibe & Ismail Küçükaylak & Mugdha Sawant & Lena Keufgens & Pia Frommelt & Kai Halwas & Katrina Broadbent & Marina Cunquero & Gustavo , 2024. "TANGO1 inhibitors reduce collagen secretion and limit tissue scarring," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    3. Kosuke Kamemura & Rio Kozono & Mizuki Tando & Misako Okumura & Daisuke Koga & Satoshi Kusumi & Kanako Tamai & Aoi Okumura & Sayaka Sekine & Daichi Kamiyama & Takahiro Chihara, 2024. "Secretion of endoplasmic reticulum protein VAPB/ALS8 requires topological inversion," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

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