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The F-box protein TIR1 is an auxin receptor

Author

Listed:
  • Nihal Dharmasiri

    (Indiana University)

  • Sunethra Dharmasiri

    (Indiana University)

  • Mark Estelle

    (Indiana University)

Abstract

The plant hormone auxin regulates diverse aspects of plant growth and development. Recent studies indicate that auxin acts by promoting the degradation of the Aux/IAA transcriptional repressors through the action of the ubiquitin protein ligase SCFTIR1. The nature of the signalling cascade that leads to this effect is not known. However, recent studies indicate that the auxin receptor and other signalling components involved in this response are soluble factors. Using an in vitro pull-down assay, we demonstrate that the interaction between transport inhibitor response 1 (TIR1) and Aux/IAA proteins does not require stable modification of either protein. Instead auxin promotes the Aux/IAA–SCFTIR1 interaction by binding directly to SCFTIR1. We further show that the loss of TIR1 and three related F-box proteins eliminates saturable auxin binding in plant extracts. Finally, TIR1 synthesized in insect cells binds Aux/IAA proteins in an auxin-dependent manner. Together, these results indicate that TIR1 is an auxin receptor that mediates Aux/IAA degradation and auxin-regulated transcription.

Suggested Citation

  • Nihal Dharmasiri & Sunethra Dharmasiri & Mark Estelle, 2005. "The F-box protein TIR1 is an auxin receptor," Nature, Nature, vol. 435(7041), pages 441-445, May.
    • Handle: RePEc:nat:nature:v:435:y:2005:i:7041:d:10.1038_nature03543
    DOI: 10.1038/nature03543
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    Cited by:

    1. Linda Lauinger & Anna Andronicos & Karin Flick & Clinton Yu & Geetha Durairaj & Lan Huang & Peter Kaiser, 2024. "Cadmium binding by the F-box domain induces p97-mediated SCF complex disassembly to activate stress response programs," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    2. Shiyun Cao & Shoukai Kang & Haibin Mao & Jiayu Yao & Liangcai Gu & Ning Zheng, 2022. "Defining molecular glues with a dual-nanobody cannabidiol sensor," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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