IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v426y2003i6966d10.1038_nature02166.html
   My bibliography  Save this article

Proteomic characterization of the human centrosome by protein correlation profiling

Author

Listed:
  • Jens S. Andersen

    (University of Southern Denmark)

  • Christopher J. Wilkinson

    (Max Planck Institute for Biochemistry)

  • Thibault Mayor

    (Max Planck Institute for Biochemistry
    California Institute of Technology)

  • Peter Mortensen

    (University of Southern Denmark)

  • Erich A. Nigg

    (Max Planck Institute for Biochemistry)

  • Matthias Mann

    (University of Southern Denmark)

Abstract

The centrosome is the major microtubule-organizing centre of animal cells and through its influence on the cytoskeleton is involved in cell shape, polarity and motility. It also has a crucial function in cell division because it determines the poles of the mitotic spindle that segregates duplicated chromosomes between dividing cells1,2,3,4,5. Despite the importance of this organelle to cell biology and more than 100 years of study, many aspects of its function remain enigmatic and its structure and composition are still largely unknown. We performed a mass-spectrometry-based proteomic analysis of human centrosomes in the interphase of the cell cycle by quantitatively profiling hundreds of proteins across several centrifugation fractions. True centrosomal proteins were revealed by both correlation with already known centrosomal proteins and in vivo localization. We identified and validated 23 novel components and identified 41 likely candidates as well as the vast majority of the known centrosomal proteins in a large background of nonspecific proteins. Protein correlation profiling permits the analysis of any multiprotein complex that can be enriched by fractionation but not purified to homogeneity.

Suggested Citation

  • Jens S. Andersen & Christopher J. Wilkinson & Thibault Mayor & Peter Mortensen & Erich A. Nigg & Matthias Mann, 2003. "Proteomic characterization of the human centrosome by protein correlation profiling," Nature, Nature, vol. 426(6966), pages 570-574, December.
    • Handle: RePEc:nat:nature:v:426:y:2003:i:6966:d:10.1038_nature02166
    DOI: 10.1038/nature02166
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature02166
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature02166?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Gayle B Collin & Jan D Marshall & Benjamin L King & Gabriella Milan & Pietro Maffei & Daniel J Jagger & Jürgen K Naggert, 2012. "The Alström Syndrome Protein, ALMS1, Interacts with α-Actinin and Components of the Endosome Recycling Pathway," PLOS ONE, Public Library of Science, vol. 7(5), pages 1-9, May.
    2. Elisabetta Zulato & Francesca Favaretto & Caterina Veronese & Stefano Campanaro & Jan D Marshall & Sara Romano & Anna Cabrelle & Gayle B Collin & Barbara Zavan & Anna S Belloni & Enrica Rampazzo & Jür, 2011. "ALMS1-Deficient Fibroblasts Over-Express Extra-Cellular Matrix Components, Display Cell Cycle Delay and Are Resistant to Apoptosis," PLOS ONE, Public Library of Science, vol. 6(4), pages 1-13, April.
    3. Lisa M Breckels & Sean B Holden & David Wojnar & Claire M Mulvey & Andy Christoforou & Arnoud Groen & Matthew W B Trotter & Oliver Kohlbacher & Kathryn S Lilley & Laurent Gatto, 2016. "Learning from Heterogeneous Data Sources: An Application in Spatial Proteomics," PLOS Computational Biology, Public Library of Science, vol. 12(5), pages 1-26, May.
    4. Helena G Dos Santos & David Abia & Robert Janowski & Gulnahar Mortuza & Michela G Bertero & Maïlys Boutin & Nayibe Guarín & Raúl Méndez-Giraldez & Alfonso Nuñez & Juan G Pedrero & Pilar Redondo & Marí, 2013. "Structure and Non-Structure of Centrosomal Proteins," PLOS ONE, Public Library of Science, vol. 8(5), pages 1-8, May.
    5. Hans J C T Wessels & Rutger O Vogel & Robert N Lightowlers & Johannes N Spelbrink & Richard J Rodenburg & Lambert P van den Heuvel & Alain J van Gool & Jolein Gloerich & Jan A M Smeitink & Leo G Nijtm, 2013. "Analysis of 953 Human Proteins from a Mitochondrial HEK293 Fraction by Complexome Profiling," PLOS ONE, Public Library of Science, vol. 8(7), pages 1-14, July.
    6. Aurélien Naldi & Romain M Larive & Urszula Czerwinska & Serge Urbach & Philippe Montcourrier & Christian Roy & Jérôme Solassol & Gilles Freiss & Peter J Coopman & Ovidiu Radulescu, 2017. "Reconstruction and signal propagation analysis of the Syk signaling network in breast cancer cells," PLOS Computational Biology, Public Library of Science, vol. 13(3), pages 1-27, March.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:426:y:2003:i:6966:d:10.1038_nature02166. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.