IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v423y2003i6937d10.1038_nature01585.html
   My bibliography  Save this article

The Srs2 helicase prevents recombination by disrupting Rad51 nucleoprotein filaments

Author

Listed:
  • Xavier Veaute

    (UMR217 CNRS/CEA)

  • Josette Jeusset

    (UMR 81126 CNRS/IGR/UPS, Institut Gustave Roussy)

  • Christine Soustelle

    (UMR217 CNRS/CEA
    UMR2167 CNRS Centre de Génétique Moléculaire)

  • Stephen C. Kowalczykowski

    (University of California)

  • Eric Le Cam

    (UMR 81126 CNRS/IGR/UPS, Institut Gustave Roussy)

  • Francis Fabre

    (UMR217 CNRS/CEA)

Abstract

Homologous recombination is a ubiquitous process with key functions in meiotic and vegetative cells for the repair of DNA breaks. It is initiated by the formation of single-stranded DNA on which recombination proteins bind to form a nucleoprotein filament that is active in searching for homology, in the formation of joint molecules and in the exchange of DNA strands1. This process contributes to genome stability but it is also potentially dangerous to cells if intermediates are formed that cannot be processed normally and thus are toxic or generate genomic rearrangements. Cells must therefore have developed strategies to survey recombination and to prevent the occurrence of such deleterious events. In Saccharomyces cerevisiae, genetic data have shown that the Srs2 helicase negatively modulates recombination2,3, and later experiments suggested that it reverses intermediate recombination structures4,5,6,7. Here we show that DNA strand exchange mediated in vitro by Rad51 is inhibited by Srs2, and that Srs2 disrupts Rad51 filaments formed on single-stranded DNA. These data provide an explanation for the anti-recombinogenic role of Srs2 in vivo and highlight a previously unknown mechanism for recombination control.

Suggested Citation

  • Xavier Veaute & Josette Jeusset & Christine Soustelle & Stephen C. Kowalczykowski & Eric Le Cam & Francis Fabre, 2003. "The Srs2 helicase prevents recombination by disrupting Rad51 nucleoprotein filaments," Nature, Nature, vol. 423(6937), pages 309-312, May.
  • Handle: RePEc:nat:nature:v:423:y:2003:i:6937:d:10.1038_nature01585
    DOI: 10.1038/nature01585
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature01585
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.

    File URL: https://libkey.io/10.1038/nature01585?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Akbar Zainu & Pauline Dupaigne & Soumya Bouchouika & Julien Cau & Julie A. J. Clément & Pauline Auffret & Virginie Ropars & Jean-Baptiste Charbonnier & Bernard Massy & Raphael Mercier & Rajeev Kumar &, 2024. "FIGNL1-FIRRM is essential for meiotic recombination and prevents DNA damage-independent RAD51 and DMC1 loading," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
    2. Guangxue Liu & Jimin Li & Boxue He & Jiaqi Yan & Jingyu Zhao & Xuejie Wang & Xiaocong Zhao & Jingyan Xu & Yeyao Wu & Simin Zhang & Xiaoli Gan & Chun Zhou & Xiangpan Li & Xinghua Zhang & Xuefeng Chen, 2023. "Bre1/RNF20 promotes Rad51-mediated strand exchange and antagonizes the Srs2/FBH1 helicases," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    3. Aviv Meir & Vivek B. Raina & Carly E. Rivera & Léa Marie & Lorraine S. Symington & Eric C. Greene, 2023. "The separation pin distinguishes the pro– and anti–recombinogenic functions of Saccharomyces cerevisiae Srs2," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:423:y:2003:i:6937:d:10.1038_nature01585. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.