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A histone H3 methyltransferase controls DNA methylation in Neurospora crassa

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  • Hisashi Tamaru

    (Institute of Molecular Biology, University of Oregon)

  • Eric U. Selker

    (Institute of Molecular Biology, University of Oregon)

Abstract

DNA methylation is involved in epigenetic processes such as X-chromosome inactivation, imprinting and silencing of transposons. We have demonstrated previously that dim-2 encodes a DNA methyltransferase that is responsible for all known cytosine methylation in Neurospora crassa. Here we report that another Neurospora gene, dim-5, is required for DNA methylation, as well as for normal growth and full fertility. We mapped dim-5 and identified it by transformation with a candidate gene. The mutant has a nonsense mutation in a SET domain of a gene related to histone methyltransferases that are involved in heterochromatin formation in other organisms. Transformation of a wild-type strain with a segment of dim-5 reactivated a silenced hph gene, apparently by ‘quelling’ of dim-5. We demonstrate that recombinant DIM-5 protein specifically methylates histone H3 and that replacement of lysine 9 in histone H3 with either a leucine or an arginine phenocopies the dim-5 mutation. We conclude that DNA methylation depends on histone methylation.

Suggested Citation

  • Hisashi Tamaru & Eric U. Selker, 2001. "A histone H3 methyltransferase controls DNA methylation in Neurospora crassa," Nature, Nature, vol. 414(6861), pages 277-283, November.
    • Handle: RePEc:nat:nature:v:414:y:2001:i:6861:d:10.1038_35104508
    DOI: 10.1038/35104508
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    Cited by:

    1. Zengyu Shao & Jiuwei Lu & Nelli Khudaverdyan & Jikui Song, 2024. "Multi-layered heterochromatin interaction as a switch for DIM2-mediated DNA methylation," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

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