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Gene expression in Pseudomonas aeruginosa biofilms

Author

Listed:
  • Marvin Whiteley

    (University of Iowa College of Medicine)

  • M. Gita Bangera

    (Harvard University)

  • Roger E. Bumgarner

    (University of Washington)

  • Matthew R. Parsek

    (Northwestern University)

  • Gail M. Teitzel

    (Northwestern University)

  • Stephen Lory

    (Harvard University)

  • E. P. Greenberg

    (University of Iowa College of Medicine)

Abstract

Bacteria often adopt a sessile biofilm lifestyle that is resistant to antimicrobial treatment1,2,3,4,5. Opportunistic pathogenic bacteria like Pseudomonas aeruginosa can develop persistent infections1,2,3. To gain insights into the differences between free-living P. aeruginosa cells and those in biofilms, and into the mechanisms underlying the resistance of biofilms to antibiotics, we used DNA microarrays. Here we show that, despite the striking differences in lifestyles, only about 1% of genes showed differential expression in the two growth modes; about 0.5% of genes were activated and about 0.5% were repressed in biofilms. Some of the regulated genes are known to affect antibiotic sensitivity of free-living P. aeruginosa. Exposure of biofilms to high levels of the antibiotic tobramycin caused differential expression of 20 genes. We propose that this response is critical for the development of biofilm resistance to tobramycin. Our results show that gene expression in biofilm cells is similar to that in free-living cells but there are a small number of significant differences. Our identification of biofilm-regulated genes points to mechanisms of biofilm resistance to antibiotics.

Suggested Citation

  • Marvin Whiteley & M. Gita Bangera & Roger E. Bumgarner & Matthew R. Parsek & Gail M. Teitzel & Stephen Lory & E. P. Greenberg, 2001. "Gene expression in Pseudomonas aeruginosa biofilms," Nature, Nature, vol. 413(6858), pages 860-864, October.
    • Handle: RePEc:nat:nature:v:413:y:2001:i:6858:d:10.1038_35101627
    DOI: 10.1038/35101627
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    Cited by:

    1. Kulyash Meiramkulova & Aliya Temirbekova & Gulnur Saspugayeva & Assel Kydyrbekova & Davud Devrishov & Zhanar Tulegenova & Karlygash Aubakirova & Nataliya Kovalchuk & Abdilda Meirbekov & Timoth Mkilima, 2021. "Performance of a Combined Treatment Approach on the Elimination of Microbes from Poultry Slaughterhouse Wastewater," Sustainability, MDPI, vol. 13(6), pages 1-12, March.
    2. Jan Böhning & Miles Graham & Suzanne C. Letham & Luke K. Davis & Ulrike Schulze & Phillip J. Stansfeld & Robin A. Corey & Philip Pearce & Abul K. Tarafder & Tanmay A. M. Bharat, 2023. "Biophysical basis of filamentous phage tactoid-mediated antibiotic tolerance in P. aeruginosa," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    3. Yunxue Guo & Kaihao Tang & Brandon Sit & Jiayu Gu & Ran Chen & Xinqi Shao & Shituan Lin & Zixian Huang & Zhaolong Nie & Jianzhong Lin & Xiaoxiao Liu & Weiquan Wang & Xinyu Gao & Tianlang Liu & Fei Liu, 2024. "Control of lysogeny and antiphage defense by a prophage-encoded kinase-phosphatase module," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    4. Yanchao Zhang & Li Zheng & Shuai Wang & Yangguo Zhao & Xiyuan Xu & Bin Han & Tianyi Hu, 2021. "Quorum Sensing Bacteria in the Phycosphere of HAB Microalgae and Their Ecological Functions Related to Cross-Kingdom Interactions," IJERPH, MDPI, vol. 19(1), pages 1-15, December.

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