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Voltage-induced membrane movement

Author

Listed:
  • Ping-Cheng Zhang

    (HHMI Center for Single Molecule Biophysics, State University of New York at Buffalo)

  • Asbed M. Keleshian

    (HHMI Center for Single Molecule Biophysics, State University of New York at Buffalo)

  • Frederick Sachs

    (HHMI Center for Single Molecule Biophysics, State University of New York at Buffalo)

Abstract

Thermodynamics predicts that transmembrane voltage modulates membrane tension1 and that this will cause movement. The magnitude and polarity of movement is governed by cell stiffness and surface potentials. Here we confirm these predictions using the atomic force microscope to dynamically follow the movement of voltage-clamped HEK293 cells2 in different ionic-strength solutions. In normal saline, depolarization caused an outward movement, and at low ionic strength an inward movement. The amplitude was proportional to voltage (about 1 nm per 100 mV) and increased with indentation depth. A simple physical model of the membrane and tip provided an estimate of the external and internal surface charge densities (-5 × 10-3 C m-2 and -18 × 10-3 C m-2, respectively). Salicylate (a negative amphiphile3) inhibited electromotility by increasing the external charge density by -15 × 10-3 C m-2. As salicylate blocks electromotility in cochlear outer hair cells at the same concentration4,5, the role of prestin as a motor protein6 may need to be reassessed.

Suggested Citation

  • Ping-Cheng Zhang & Asbed M. Keleshian & Frederick Sachs, 2001. "Voltage-induced membrane movement," Nature, Nature, vol. 413(6854), pages 428-432, September.
    • Handle: RePEc:nat:nature:v:413:y:2001:i:6854:d:10.1038_35096578
    DOI: 10.1038/35096578
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    Cited by:

    1. Lucia, Umberto, 2014. "Thermodynamic approach to nano-properties of cell membrane," Physica A: Statistical Mechanics and its Applications, Elsevier, vol. 407(C), pages 185-191.
    2. Carlos A. Z. Bassetto & Juergen Pfeffermann & Rohit Yadav & Simon Strassgschwandtner & Toma Glasnov & Francisco Bezanilla & Peter Pohl, 2024. "Photolipid excitation triggers depolarizing optocapacitive currents and action potentials," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    3. Tao Chen & Narain Karedla & Jörg Enderlein, 2024. "Measuring sub-nanometer undulations at microsecond temporal resolution with metal- and graphene-induced energy transfer spectroscopy," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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