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Measuring sub-nanometer undulations at microsecond temporal resolution with metal- and graphene-induced energy transfer spectroscopy

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  • Tao Chen

    (Georg August University)

  • Narain Karedla

    (The Rosalind Franklin Institute
    University of Oxford)

  • Jörg Enderlein

    (Georg August University
    Universitätsmedizin Göttingen)

Abstract

Out-of-plane fluctuations, also known as stochastic displacements, of biological membranes play a crucial role in regulating many essential life processes within cells and organelles. Despite the availability of various methods for quantifying membrane dynamics, accurately quantifying complex membrane systems with rapid and tiny fluctuations, such as mitochondria, remains a challenge. In this work, we present a methodology that combines metal/graphene-induced energy transfer (MIET/GIET) with fluorescence correlation spectroscopy (FCS) to quantify out-of-plane fluctuations of membranes with simultaneous spatiotemporal resolution of approximately one nanometer and one microsecond. To validate the technique and spatiotemporal resolution, we measure bending undulations of model membranes. Furthermore, we demonstrate the versatility and applicability of MIET/GIET-FCS for studying diverse membrane systems, including the widely studied fluctuating membrane system of human red blood cells, as well as two unexplored membrane systems with tiny fluctuations, a pore-spanning membrane, and mitochondrial inner/outer membranes.

Suggested Citation

  • Tao Chen & Narain Karedla & Jörg Enderlein, 2024. "Measuring sub-nanometer undulations at microsecond temporal resolution with metal- and graphene-induced energy transfer spectroscopy," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-45822-x
    DOI: 10.1038/s41467-024-45822-x
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    References listed on IDEAS

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    1. C. Monzel & D. Schmidt & C. Kleusch & D. Kirchenbüchler & U. Seifert & A-S Smith & K. Sengupta & R. Merkel, 2015. "Measuring fast stochastic displacements of bio-membranes with dynamic optical displacement spectroscopy," Nature Communications, Nature, vol. 6(1), pages 1-8, November.
    2. Ping-Cheng Zhang & Asbed M. Keleshian & Frederick Sachs, 2001. "Voltage-induced membrane movement," Nature, Nature, vol. 413(6854), pages 428-432, September.
    3. Silvia N. Kariuki & Alejandro Marin-Menendez & Viola Introini & Benjamin J. Ravenhill & Yen-Chun Lin & Alex Macharia & Johnstone Makale & Metrine Tendwa & Wilfred Nyamu & Jurij Kotar & Manuela Carrasq, 2020. "Red blood cell tension protects against severe malaria in the Dantu blood group," Nature, Nature, vol. 585(7826), pages 579-583, September.
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