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Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

Author

Listed:
  • Sayda M. Elbashir

    (Max-Planck-Institute for Biophysical Chemistry)

  • Jens Harborth

    (Max-Planck-Institute for Biophysical Chemistry)

  • Winfried Lendeckel

    (Max-Planck-Institute for Biophysical Chemistry)

  • Abdullah Yalcin

    (Max-Planck-Institute for Biophysical Chemistry)

  • Klaus Weber

    (Max-Planck-Institute for Biophysical Chemistry)

  • Thomas Tuschl

    (Max-Planck-Institute for Biophysical Chemistry)

Abstract

RNA interference (RNAi) is the process of sequence-specific, post-transcriptional gene silencing in animals and plants, initiated by double-stranded RNA (dsRNA) that is homologous in sequence to the silenced gene1,2,3,4. The mediators of sequence-specific messenger RNA degradation are 21- and 22-nucleotide small interfering RNAs (siRNAs) generated by ribonuclease III cleavage from longer dsRNAs5,6,7,8,9. Here we show that 21-nucleotide siRNA duplexes specifically suppress expression of endogenous and heterologous genes in different mammalian cell lines, including human embryonic kidney (293) and HeLa cells. Therefore, 21-nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and may eventually be used as gene-specific therapeutics.

Suggested Citation

  • Sayda M. Elbashir & Jens Harborth & Winfried Lendeckel & Abdullah Yalcin & Klaus Weber & Thomas Tuschl, 2001. "Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells," Nature, Nature, vol. 411(6836), pages 494-498, May.
    • Handle: RePEc:nat:nature:v:411:y:2001:i:6836:d:10.1038_35078107
    DOI: 10.1038/35078107
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    Cited by:

    1. Ana M Rojas & Anna Santamaria & Rainer Malik & Thomas Skøt Jensen & Roman Körner & Ian Morilla & David de Juan & Martin Krallinger & Daniel Aaen Hansen & Robert Hoffmann & Jonathan Lees & Adam Reid & , 2012. "Uncovering the Molecular Machinery of the Human Spindle—An Integration of Wet and Dry Systems Biology," PLOS ONE, Public Library of Science, vol. 7(3), pages 1-16, March.
    2. Joseph Nsengimana & Lander Bauters & Annelies Haegeman & Godelieve Gheysen, 2013. "Silencing of Mg - pat-10 and Mg - unc-87 in the Plant Parasitic Nematode Meloidogyne graminicola Using siRNAs," Agriculture, MDPI, vol. 3(3), pages 1-12, September.
    3. Song Cui & Eunseog Youn & Joohyun Lee & Stephan J Maas, 2014. "An Improved Systematic Approach to Predicting Transcription Factor Target Genes Using Support Vector Machine," PLOS ONE, Public Library of Science, vol. 9(4), pages 1-9, April.

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