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Role for a bidentate ribonuclease in the initiation step of RNA interference

Author

Listed:
  • Emily Bernstein

    (Cold Spring Harbor Laboratory
    Graduate Program in Genetics, State University of New York at Stony Brook)

  • Amy A. Caudy

    (Cold Spring Harbor Laboratory
    Watson School of Biological Sciences)

  • Scott M. Hammond

    (Cold Spring Harbor Laboratory
    Genetica)

  • Gregory J. Hannon

    (Cold Spring Harbor Laboratory)

Abstract

RNA interference (RNAi) is the mechanism through which double-stranded RNAs silence cognate genes1,2,3,4,5. In plants, this can occur at both the transcriptional and the post-transcriptional levels1,2,5; however, in animals, only post-transcriptional RNAi has been reported to date. In both plants and animals, RNAi is characterized by the presence of RNAs of about 22 nucleotides in length that are homologous to the gene that is being suppressed6,7,8. These 22-nucleotide sequences serve as guide sequences that instruct a multicomponent nuclease, RISC, to destroy specific messenger RNAs6. Here we identify an enzyme, Dicer, which can produce putative guide RNAs. Dicer is a member of the RNase III family of nucleases that specifically cleave double-stranded RNAs, and is evolutionarily conserved in worms, flies, plants, fungi and mammals. The enzyme has a distinctive structure, which includes a helicase domain and dual RNase III motifs. Dicer also contains a region of homology to the RDE1/QDE2/ARGONAUTE family that has been genetically linked to RNAi9,10.

Suggested Citation

  • Emily Bernstein & Amy A. Caudy & Scott M. Hammond & Gregory J. Hannon, 2001. "Role for a bidentate ribonuclease in the initiation step of RNA interference," Nature, Nature, vol. 409(6818), pages 363-366, January.
    • Handle: RePEc:nat:nature:v:409:y:2001:i:6818:d:10.1038_35053110
    DOI: 10.1038/35053110
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    Cited by:

    1. Felix K. F. Kommoss & Anne-Sophie Chong & Anne-Laure Chong & Elke Pfaff & David T. W. Jones & Laura S. Hiemcke-Jiwa & Lennart A. Kester & Uta Flucke & Manfred Gessler & Daniel Schrimpf & Felix Sahm & , 2023. "Genomic characterization of DICER1-associated neoplasms uncovers molecular classes," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    2. Katerina Gkirtzou & Ioannis Tsamardinos & Panagiotis Tsakalides & Panayiota Poirazi, 2010. "MatureBayes: A Probabilistic Algorithm for Identifying the Mature miRNA within Novel Precursors," PLOS ONE, Public Library of Science, vol. 5(8), pages 1-14, August.
    3. Bin Liu & Longyun Fang & Fule Liu & Xiaolong Wang & Junjie Chen & Kuo-Chen Chou, 2015. "Identification of Real MicroRNA Precursors with a Pseudo Structure Status Composition Approach," PLOS ONE, Public Library of Science, vol. 10(3), pages 1-20, March.
    4. Justine M Pompey & Bardees Foda & Upinder Singh, 2015. "A Single RNaseIII Domain Protein from Entamoeba histolytica Has dsRNA Cleavage Activity and Can Help Mediate RNAi Gene Silencing in a Heterologous System," PLOS ONE, Public Library of Science, vol. 10(7), pages 1-21, July.
    5. Trung Duc Nguyen & Tam Anh Trinh & Sheng Bao & Tuan Anh Nguyen, 2022. "Secondary structure RNA elements control the cleavage activity of DICER," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    6. Marta Garcia-Montojo & Saeed Fathi & Cyrus Rastegar & Elena Rita Simula & Tara Doucet-O’Hare & Y. H. Hank Cheng & Rachel P. M. Abrams & Nicholas Pasternack & Nasir Malik & Muzna Bachani & Brianna Disa, 2024. "TDP-43 proteinopathy in ALS is triggered by loss of ASRGL1 and associated with HML-2 expression," Nature Communications, Nature, vol. 15(1), pages 1-24, December.
    7. Bidur Paudel & Si-Yeon Jeong & Carolina Pena Martinez & Alexis Rickman & Ashley Haluck-Kangas & Elizabeth T. Bartom & Kristina Fredriksen & Amira Affaneh & John A. Kessler & Joseph R. Mazzulli & Andre, 2024. "Death Induced by Survival gene Elimination (DISE) correlates with neurotoxicity in Alzheimer’s disease and aging," Nature Communications, Nature, vol. 15(1), pages 1-19, December.

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