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Total silencing by intron-spliced hairpin RNAs

Author

Listed:
  • Neil A. Smith

    (CSIRO Plant Industry)

  • Surinder P. Singh

    (CSIRO Plant Industry)

  • Ming-Bo Wang

    (CSIRO Plant Industry)

  • Peter A. Stoutjesdijk

    (CSIRO Plant Industry)

  • Allan G. Green

    (CSIRO Plant Industry)

  • Peter M. Waterhouse

    (CSIRO Plant Industry)

Abstract

Post-transcriptional gene silencing (PTGS), a sequence-specific RNA degradation mechanism inherent in many life-forms, can be induced in plants by transforming them with either antisense1 or co-suppression2 constructs, but typically this results in only a small proportion of silenced individuals. Here we show that gene constructs encoding intron-spliced RNA with a hairpin structure can induce PTGS with almost 100% efficiency when directed against viruses or endogenous genes. These constructs could prove valuable in reverse genetics, genomics, engineering of metabolic pathways and protection against pathogens.

Suggested Citation

  • Neil A. Smith & Surinder P. Singh & Ming-Bo Wang & Peter A. Stoutjesdijk & Allan G. Green & Peter M. Waterhouse, 2000. "Total silencing by intron-spliced hairpin RNAs," Nature, Nature, vol. 407(6802), pages 319-320, September.
    • Handle: RePEc:nat:nature:v:407:y:2000:i:6802:d:10.1038_35030305
    DOI: 10.1038/35030305
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    Cited by:

    1. Daai Zhang & Chengcheng Zhong & Neil A. Smith & Robert de Feyter & Ian K. Greaves & Steve M. Swain & Ren Zhang & Ming-Bo Wang, 2022. "Nucleotide mismatches prevent intrinsic self-silencing of hpRNA transgenes to enhance RNAi stability in plants," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    2. Dana ŠAFÁŘOVÁ & Pavel BRÁZDA & Milan NAVRÁTIL, 2014. "Effect of artificial dsRNA on infection of pea plants by Pea seed-borne mosaic virus," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 50(2), pages 105-108.

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