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Effect of artificial dsRNA on infection of pea plants by Pea seed-borne mosaic virus

Author

Listed:
  • Dana ŠAFÁŘOVÁ

    (Department of Cell Biology and Genetics, Faculty of Science, Palacký University Olomouc, Olomouc, Czech Republic)

  • Pavel BRÁZDA

    (Department of Cell Biology and Genetics, Faculty of Science, Palacký University Olomouc, Olomouc, Czech Republic)

  • Milan NAVRÁTIL

    (Department of Cell Biology and Genetics, Faculty of Science, Palacký University Olomouc, Olomouc, Czech Republic)

Abstract

The effect of direct application of artificial dsRNA molecules derived from the viral coat protein gene and the possibility of RNA interference induction leading to protection against Pea seed borne mosaic virus (PSbMV) have been investigated. Effectivity of virus transmission was not afected by the dsRNA treatment. Simultaneous application of dsRNA and virus inoculation as well as dsRNA application one day before PSbMV inoculation led to a significant short-term decrease in the PSbMV concentration in experimental pea plants. Either the curative effect of the artificial dsRNA biolistic application was not noticed.

Suggested Citation

  • Dana ŠAFÁŘOVÁ & Pavel BRÁZDA & Milan NAVRÁTIL, 2014. "Effect of artificial dsRNA on infection of pea plants by Pea seed-borne mosaic virus," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 50(2), pages 105-108.
    • Handle: RePEc:caa:jnlcjg:v:50:y:2014:i:2:id:120-2013-cjgpb
    DOI: 10.17221/120/2013-CJGPB
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    References listed on IDEAS

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    1. Neil A. Smith & Surinder P. Singh & Ming-Bo Wang & Peter A. Stoutjesdijk & Allan G. Green & Peter M. Waterhouse, 2000. "Total silencing by intron-spliced hairpin RNAs," Nature, Nature, vol. 407(6802), pages 319-320, September.
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