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A chromatin remodelling complex involved in transcription and DNA processing

Author

Listed:
  • Xuetong Shen

    (Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health)

  • Gaku Mizuguchi

    (Kyoto University Graduate School of Biostudies)

  • Ali Hamiche

    (Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health)

  • Carl Wu

    (Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health)

Abstract

The packaging of the eukaryotic genome in chromatin presents barriers that restrict the access of enzymes that process DNA1,2. To overcome these barriers, cells possess a number of multi-protein, ATP-dependent chromatin remodelling complexes, each containing an ATPase subunit from the SNF2/SWI2 superfamily3,4. Chromatin remodelling complexes function by increasing nucleosome mobility and are clearly implicated in transcription5,6,7. Here we have analysed SNF2/SWI2- and ISWI-related proteins to identify remodelling complexes that potentially assist other DNA transactions. We purified a complex from Saccharomyces cerevisiae that contains the Ino80 ATPase8. The INO80 complex contains about 12 polypeptides including two proteins related to the bacterial RuvB DNA helicase9,10,11, which catalyses branch migration of Holliday junctions. The purified complex remodels chromatin, facilitates transcription in vitro and displays 3′ to 5′ DNA helicase activity. Mutants of ino80 show hypersensitivity to agents that cause DNA damage, in addition to defects in transcription8. These results indicate that chromatin remodelling driven by the Ino80 ATPase may be connected to transcription as well as DNA damage repair.

Suggested Citation

  • Xuetong Shen & Gaku Mizuguchi & Ali Hamiche & Carl Wu, 2000. "A chromatin remodelling complex involved in transcription and DNA processing," Nature, Nature, vol. 406(6795), pages 541-544, August.
    • Handle: RePEc:nat:nature:v:406:y:2000:i:6795:d:10.1038_35020123
    DOI: 10.1038/35020123
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    Cited by:

    1. Cuige Zhu & Mari Iwase & Ziqian Li & Faliang Wang & Annabel Quinet & Alessandro Vindigni & Jieya Shao, 2022. "Profilin-1 regulates DNA replication forks in a context-dependent fashion by interacting with SNF2H and BOD1L," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    2. Lidice González & Daniel Kolbin & Christian Trahan & Célia Jeronimo & François Robert & Marlene Oeffinger & Kerry Bloom & Stephen W. Michnick, 2023. "Adaptive partitioning of a gene locus to the nuclear envelope in Saccharomyces cerevisiae is driven by polymer-polymer phase separation," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

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