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Enzymatic production of biohydrogen

Author

Listed:
  • Jonathan Woodward

    (Oak Ridge National Laboratory)

  • Mark Orr

    (Oak Ridge National Laboratory)

  • Kimberley Cordray

    (Oak Ridge National Laboratory)

  • Elias Greenbaum

    (Oak Ridge National Laboratory)

Abstract

Although in theory the amount of hydrogen that could be generated from renewable sources of energy such as cellulose (a polymer of glucose) is vast1, only 16–24% of the maximum stoichiometric yield of hydrogen from glucose (about 12 mol H2 per mol glucose) is typically achieved by biological methods2. Here we show that the enzymes of the oxidative pentose phosphate cycle3,4,5 can be coupled to hydrogenase purified from the bacterium Pyrococcus furiosus, one of only a few hydrogenases that use NADP+ as the electron carrier6, to generate 11.6 mol H2 per mol glucose-6-phosphate. Hydrogen produced by this pathway is the major product, unlike that produced by intermediate metabolic pathways of bacterial fermentation, and therefore has important practical implications for biohydrogen production7.

Suggested Citation

  • Jonathan Woodward & Mark Orr & Kimberley Cordray & Elias Greenbaum, 2000. "Enzymatic production of biohydrogen," Nature, Nature, vol. 405(6790), pages 1014-1015, June.
    • Handle: RePEc:nat:nature:v:405:y:2000:i:6790:d:10.1038_35016633
    DOI: 10.1038/35016633
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    Citations

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    Cited by:

    1. Samir Bensaid & Bernardo Ruggeri & Guido Saracco, 2015. "Development of a Photosynthetic Microbial Electrochemical Cell (PMEC) Reactor Coupled with Dark Fermentation of Organic Wastes: Medium Term Perspectives," Energies, MDPI, vol. 8(1), pages 1-31, January.
    2. Sołowski, Gaweł & Shalaby, Marwa.S. & Abdallah, Heba & Shaban, Ahmed.M. & Cenian, Adam, 2018. "Production of hydrogen from biomass and its separation using membrane technology," Renewable and Sustainable Energy Reviews, Elsevier, vol. 82(P3), pages 3152-3167.
    3. Harish, B.S & Janaki Ramaiah, M. & Babu Uppuluri, Kiran, 2015. "Bioengineering strategies on catalysis for the effective production of renewable and sustainable energy," Renewable and Sustainable Energy Reviews, Elsevier, vol. 51(C), pages 533-547.
    4. Patel, Anil Kumar & Vaisnav, Neha & Mathur, Anshu & Gupta, Ravi & Tuli, Deepak Kumar, 2016. "Whey waste as potential feedstock for biohydrogen production," Renewable Energy, Elsevier, vol. 98(C), pages 221-225.
    5. Yiyang Liu & Jinze Liu & Hongzhen He & Shanru Yang & Yixiao Wang & Jin Hu & Huan Jin & Tianxiang Cui & Gang Yang & Yong Sun, 2021. "A Review of Enhancement of Biohydrogen Productions by Chemical Addition Using a Supervised Machine Learning Method," Energies, MDPI, vol. 14(18), pages 1-16, September.
    6. Packer, Mike, 2009. "Algal capture of carbon dioxide; biomass generation as a tool for greenhouse gas mitigation with reference to New Zealand energy strategy and policy," Energy Policy, Elsevier, vol. 37(9), pages 3428-3437, September.
    7. Azwar, M.Y. & Hussain, M.A. & Abdul-Wahab, A.K., 2014. "Development of biohydrogen production by photobiological, fermentation and electrochemical processes: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 31(C), pages 158-173.
    8. Yiyang Liu & Jingluo Min & Xingyu Feng & Yue He & Jinze Liu & Yixiao Wang & Jun He & Hainam Do & Valérie Sage & Gang Yang & Yong Sun, 2020. "A Review of Biohydrogen Productions from Lignocellulosic Precursor via Dark Fermentation: Perspective on Hydrolysate Composition and Electron-Equivalent Balance," Energies, MDPI, vol. 13(10), pages 1-27, May.

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