IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v403y2000i6769d10.1038_35000615.html
   My bibliography  Save this article

Evidence for stabilizing selection in a eukaryotic enhancer element

Author

Listed:
  • Michael Z. Ludwig

    (University of Chicago)

  • Casey Bergman

    (University of Chicago)

  • Nipam H. Patel

    (MC1028, N-101)

  • Martin Kreitman

    (University of Chicago)

Abstract

Eukaryotic gene expression is mediated by compact cis-regulatory modules, or enhancers, which are bound by specific sets of transcription factors1. The combinatorial interaction of these bound transcription factors determines time- and tissue-specific gene activation or repression. The even-skipped stripe 2 element controls the expression of the second transverse stripe of even-skipped messenger RNA in Drosophila melanogaster embryos, and is one of the best characterized eukaryotic enhancers2,3,4. Although even-skipped stripe 2 expression is strongly conserved in Drosophila, the stripe 2 element itself has undergone considerable evolutionary change in its binding-site sequences and the spacing between them. We have investigated this apparent contradiction, and here we show that two chimaeric enhancers, constructed by swapping the 5′ and 3′ halves of the native stripe 2 elements of two species, no longer drive expression of a reporter gene in the wild-type pattern. Sequence differences between species have functional consequences, therefore, but they are masked by other co-evolved differences. On the basis of these results, we present a model for the evolution of eukaryotic regulatory sequences.

Suggested Citation

  • Michael Z. Ludwig & Casey Bergman & Nipam H. Patel & Martin Kreitman, 2000. "Evidence for stabilizing selection in a eukaryotic enhancer element," Nature, Nature, vol. 403(6769), pages 564-567, February.
    • Handle: RePEc:nat:nature:v:403:y:2000:i:6769:d:10.1038_35000615
    DOI: 10.1038/35000615
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/35000615
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/35000615?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Pradipta Ray & Suyash Shringarpure & Mladen Kolar & Eric P Xing, 2008. "CSMET: Comparative Genomic Motif Detection via Multi-Resolution Phylogenetic Shadowing," PLOS Computational Biology, Public Library of Science, vol. 4(6), pages 1-20, June.
    2. Lauren A. Choate & Gilad Barshad & Pierce W. McMahon & Iskander Said & Edward J. Rice & Paul R. Munn & James J. Lewis & Charles G. Danko, 2021. "Multiple stages of evolutionary change in anthrax toxin receptor expression in humans," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    3. Armita Nourmohammad & Michael Lässig, 2011. "Formation of Regulatory Modules by Local Sequence Duplication," PLOS Computational Biology, Public Library of Science, vol. 7(10), pages 1-12, October.
    4. Iksoo Huh & Isabel Mendizabal & Taesung Park & Soojin V Yi, 2018. "Functional conservation of sequence determinants at rapidly evolving regulatory regions across mammals," PLOS Computational Biology, Public Library of Science, vol. 14(10), pages 1-21, October.
    5. Mathilde Paris & Tommy Kaplan & Xiao Yong Li & Jacqueline E Villalta & Susan E Lott & Michael B Eisen, 2013. "Extensive Divergence of Transcription Factor Binding in Drosophila Embryos with Highly Conserved Gene Expression," PLOS Genetics, Public Library of Science, vol. 9(9), pages 1-18, September.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:403:y:2000:i:6769:d:10.1038_35000615. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.